Cloning of Two New cry Genes from Bacillus thuringiensis subsp. wuhanensis Strain |
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| Authors: | White-Shang Kuo Jong-Huon Lin Ching-Chou Tzeng Shui-Shang Kao Kin-Fu Chak |
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| Affiliation: | (1) Institute of Biochemistry, National Yang Ming University, Shih-Pai, Taipei, Taiwan 11221, Republic of China , TW;(2) Taiwan Agricultural Chemicals and Toxic Substances Research Institute, Taichung Hsien 41301, Republic of China , TW |
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| Abstract: | With PCR products as probes, we have cloned two new cry-type genes from Bacillus thuringiensis subsp. wuhanensis. The deduced amino acid sequence of the first clone is 77.3% identical to Cry1Ga1. The deduced protein sequence of the second
clone is 69.8–78.7% identical to that of Cry1B group. The nomenclature assignment of these two clones is, therefore, named
Cry1Gb1 and Cry1Bd1, respectively. The Cry1Bd1 is toxic to Plutella xylostella larvae, and the Cry1Gb1 is toxic to Pieris rapae larvae.
Received: 2 August 1999 / Accepted: 18 October 1999 |
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