RRLC-Q-TOF-MS法研究人参皂苷Rh1对映异构体在大鼠体内的药代动力学行为

建立了高分离快速液相色谱-四极杆-飞行时间质谱(RRLC-Q-TOF-MS)法同时分离人参皂苷Rh1对映异构体，并研究其在大鼠体内的药代动力学行为。实验采用Agilent SB C18色谱柱，流动相A为水-乙腈（90∶10，V∶V）溶液，流动相B为水-乙腈（10∶90，V∶V）溶液，两种流动相内均含15 mmol/L甲酸铵，流速0.3 mL/min，进样量5 μL，二元线性梯度洗脱分离，在电喷雾负离子模式下进行质谱检测。结果表明，20-(S)和20-(R)人参皂苷Rh1的定量线性范围分别为0.24~39.00 mg/L和0.035~7.80 mg/L，定量限分别为0.20和0.03 mg/L，方法的检测限（S/N=3）分别为0.10和0.02 mg/L。精确度与精密度结果显示：两者的日内精确度分别为89.54%~95.79%和88.76%~94.33%，相对偏差小于5%；日间精确度分别为88.16%~92.41%和88.49%~94.35%，相对偏差小于5%。低、中、高3个浓度的提取回收率均大于90%。该方法可用于分离测定20-(S)和20-(R)人参皂苷Rh1，从而进行人参皂苷Rh1对映异构体在大鼠体内的药代动力学研究。

The Pharmacokinetics Studies of ginsenoside Rh1 Enantiomers in Rats by RRLC-Q-TOF-MS

A method of rapid resolution liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (RRLC-Q-TOF-MS) was developed for the simultaneous determination ginsenoside Rh1 enantiomers，and the pharmacokinetics of rats were studied. 20-(S) and 20-(R) ginsenoside Rh1 were separated on Agilent SB C18 column, using water acetonitrile（90∶10，V∶V）and water-acetonitrile（10∶90，V∶V），including 15 mmol/L ammonium formate as mobile phase A and B. The flow rate was 0.3 mL/min, and the injection volume was 5 μL. Determination was carried out by Q-TOF-MS in negative ion mode. The results show that the linearity of 20-(S) and 20-(R) ginsenoside Rh1 are 0.24-39.00 mg/L and 0.035-7.80 mg/L， respectively. The limits of quantification (LOQ) are 0.20 and 0.03 mg/L， the limits of detection (S/N=3) are 0.10 and 0.02 mg/L， respectively. The intra-day precisions are 89.54%-95.79% and 88.76%-94.33%, and relative deviations are less than 5%. The inter-day precisions are 88.16%-92.41% and 88.49%-94.35%, and the relative deviations are less than 5%. The recoveries of low, medium, and high concentrations are more than 90%. The method can be applied in separation and the pharmacokinetics studies of ginsenoside Rh1 enantiomers in rats.