次级淋巴组织趋化因子检测胃癌淋巴结微转移

目的]探讨次级淋巴组织趋化因子（SLC）mRNA联合检测预测淋巴结微转移对胃癌病人的临床意义。方法]分别用SLCmRNA RT-PCR方法、细胞角蛋白CK20免疫组化染色法、常规病理学方法检测2003年至2006年间手术的35例胃癌病人的523个淋巴结。结果]14例胃癌患者63个淋巴结免疫组化和常规组织学检查未证实淋巴结转移，而SLCmRNA联合细胞角蛋白CK20免疫染色以及常规组织学检查可更准确地发现淋巴结微转移灶，有利于判断胃癌病人的预后。

Secondary Lymphoid Tissue Chemokine mRNA Determination of Lymph Node Micrometastasis in Patients with Gastric Cancer

Purpose] To explore the significance of combined measurement of secondary lymphoid tissue chemokine mRNA for prediction micrometastasis of lymph nodes in patients with gastric cancer. Methods] Five hundred and twenty-three lymph nodes removed from 35 cases with gastric cancer during 2003 to 2008 were examined by immunohistochemistry of cytokeratin and SLCmRNA RT-PCR. Results] In 14 cases (63 lymph nodes) with gastric cancer, expression of SLCmRNA was reduced suggesting unconfirmed micrometastasis with immunohistochmical method and routine pathological method. The incidence of micrometastasis in undifferentiated carcinoma was higher than that in differentiated carcinoma. Conclusion] Detection of lymph node micrometastasis and accuracy prediction of prognosis in patients with gastric cancer should be greatly enhanced if cytokeratin-specific immunohistochemstry, SLCmRNA and routine histopathological examination of lymph nodes are all adopted.