白芍总甙对B淋巴细胞增殖和白介素1生成的调节作用

白芍总甙（ＴＧＰ）对脂多糖（ＬＰＳ）诱导的小鼠脾淋巴细胞增殖反应的量效曲线呈钟形，用贴壁法除去脾细胞中巨噬细胞（ＭΦ）或加入１０μｍｏｌ·Ｌ１吲哚美辛（Ｉｎｄ）可使ＴＧＰ量效曲线的下降支消失，再加５％同系小鼠腹腔ＭΦ中或前列腺素Ｅ２（ＰＧＥ２）０．０２２０μｍｏｌ·Ｌ１可使量效曲线下降支再现．同步检测ＴＧＰ对ＬＰＳ诱导大鼠腹腔ＭΦ产生ＰＧＥ２与白介素１（ＩＬ１）．结果表明，ＴＧＰ０．５－３ｌ２．５μｇ·ｍＬ１对ＬＰＳ诱导的ＩＬ－１产生曲线呈钟形．而ＴＧＰＬＰＳ的ＰＧＥ２产生曲线呈浓度依赖性地增高；在１２．５－３１２．５μｇ·ｍＬＴＧＰ范围内，１０μｍｏｌ·Ｌ１Ｉｎｄ可使高浓度ＴＣＰＬＰＳ的ＩＬ－１释放曲线明显抬高。提示ＴＧＰ对ＬＰＳ诱导的Ｂ细胞增殖反应和ＩＬ－１诱生的负调节作用都与其促进ＭΦ释放ＰＧＥ２有关．

Modulatory effects of total glucosides of paeony on B lymphocyte proliferation and interleukin 1 production

The negatively regulatory mecha-nisms of total glucosides of paeony(TGP) on lipo-polysaccharide(LPS)-induced lymphocyte proliferation in mouse spleen and interleukin 1(IL-1) pro-duction of rat peritoneal macrophages(M)were stu-died. The descending limb of concentration-effectcurve of splenocyte proliferation disappeared whenindometacin(Ind,10 umol·L1) was added orM was depleted from the cuIture and reappearedWhen 5% of M or prostaglandin E2(PGE2,0.02-20umol·L1) was added again. The results suggestedthat the negatively regulatory effects of TGP onLpS induced proliferation of splenocytes in mice weremediated by the PGE2 production of M.The effects of TGP on LPS-induccd IL-1andPCE2 prduction from rat M were studiedsimultaneously. The production curve of IL-1 seemsto be bell-shaped,but that of PGE2 was enhanced bvTGP(0.5-312.5 mg·L1)The results suggested thatthe negatively regulatory effect of TGP on LPS-in-duced IL-1 production was also mediated by thePGE2 production of M.