二甲基亚砜对原代培养大鼠肝细胞醋氨酚损伤的影响

醋氨酚（ＡＡＰ）引起肝细胞损伤时，肝细胞还原型谷胱甘肽（６ＳＨ）含量下降，胞浆游离Ｃａ２＋浓度（［Ｃａ２＋］）升高二甲亚砜（ＤＭＳＯ）对ＡＡＰ肝细胞损伤有明显的保护作用。对轻度损伤能完全拮抗，ＤＭＳＯ对ＧＳＨ含量下降有明显的拮抗作用，当ＧＳＮ维持在一定水平时，ＡＡＰ不引起［Ｃａ２＋］升高。提示ＤＭＳＯ可能通过保护ＧＳＨ等巯基物质而发挥拮抗ＡＡＰ肝细胞损伤的作用

Influences of dimethyl sulfoxide on acetaminophen-induced injury of rat hepatocytes in primary culture1

In this paper we described the protec-tive effects of dimethyl sulfoxide(DMSO)againstacetaminophen(AAP)-induced hepatotoxicity and an-alvsed its mechanism The results showed that in mo-nolayer-culture condition,DMSO could significantlyantagonise AAP-induced intracellular glutathione(GSH)depletion and damage of integrity of plasmicmembrane of rat hepatocytes. By direct determinationof GSH content and cvtosolic free calcium concentration([Ca2 ])of suspension-cultured hepatocytessynchronously,we proved that AAP could cause GSHdepletion and elevation of [Ca2 ] in hepatocvtes. whenGSH depletion was partially antagonised bv DMSOand GSH reached a level of about 63% of the normal.the elevation of [Ca2 ]could be completelyantagonised This indicated the antagonization of ele-vation of [Ca2 ]may be linked with the prevention of-thiol depletion in hepatocyte.