| 12（R）羟基二十碳四烯酸与大鼠肾皮质细胞膜的特异结合 |
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| 引用本文： | 翁福海 颜庭. 12（R）羟基二十碳四烯酸与大鼠肾皮质细胞膜的特异结合[J]. 中国药理学与毒理学杂志, 1994, 8(2): 157-158 |
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| 作者姓名： | 翁福海 颜庭 |
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| 作者单位： | 天津医学院药理教研室 |
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| 摘 要： | １２（Ｒ）羟基二十碳四烯酸与大鼠肾皮质细胞膜的特异结合翁福海，颜庭（天津医学院药理教研室，天津３０００７０）１２（Ｒ）羟基二十碳四烯酸［１２（Ｒ）－ｈｙｄｒｏｘｙｅｉｃｏｓａｔｅｔｒａ－ｅｎｏｉｃａｃｉｄ，１２（Ｒ）ＨＥＴＥ］是花生四烯酸经细胞色素Ｐ...
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| 关 键 词： | 12（R）羟基二十碳四烯酸；受体；放射配位体测定；白三烯；花生四烯酸类；细胞色素P450 |
Specific binding of 12(R)-hydroxyeicosatetraenoic acid to rat kidney cortex membrane |
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| WENG Fu-Hai,YENG Ting. Specific binding of 12(R)-hydroxyeicosatetraenoic acid to rat kidney cortex membrane[J]. Chinese Journal of Pharmacology and Toxicology, 1994, 8(2): 157-158 |
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| Authors: | WENG Fu-Hai YENG Ting |
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| Abstract: | With a radioligand binding assay,theresults showed that there may be a 12(R)-hydroxy-eicosatetraenoic acid (12(R)HETE) receptor in the cortex membrane of rat kidney .It was saturable ,specific and rever-skble ,A linear Scatchard plot was obtained,with a Kd of 20.0±s 0.7nmol·L-1 and a Bmax of 179±s 11 pmol· mg-1.LTB4 was able to competitive replacement for 12(R)HETEreceptor binding,it yielded a Ki value of 60.2±s 0.9 nmol·L-1 that was about 3 fold affinity less than that of acids;cytochrome P45012(R)HETE receptor binding .None metaboite of 12(R)HETE was found during kidney perfusion ,detected by RP-HPLC and lipuid scintillation.So it is suggested that thevalues of Kd and Bmax havent been interfered by itsmetabolites. |
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| Keywords: | R)-hydroxyeicosatetraenoic acid receptors radioligand assay leukotriene arachidonicacids cytochrome P450 |
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