广东人群XRCC1-Arg399Gln多态性与宫颈癌风险相关性研究

目的研究广东人群X线修复交叉互补基因1（XRCC1）-Arg399Gln多态性与宫颈癌发病风险的关系。方法采用病例-对照研究方法，聚合酶链反应-限制性片段长度多态性（PCR—RFLP）检测广东地区162例宫颈癌病人及年龄相匹配的183例正常人群XRCC1-Arg399Gln基因型分布频率及其与宫颈癌风险及患者临床病理特征的关系。结果病例组和对照组Arg399Gln基因型Arg／Arg、Arg／Gln和Gln／Gln频率分布无统计学意义，Arg399Gln多态性不增加个体患宫颈癌的风险；但Arg399Gln多态性与宫颈癌临床分期有关（P〈0．05），Are／Gin和Gln／Gln基因型在宫颈癌Ⅲ期患者中频率显著高于Arg／Arg基因型，这一结果提示ArgS399GIn多态性可能与宫颈癌预后有关。结论XRCC1-Arg399Gln多态性与宫颈癌发生无相关性，但与宫颈癌临床分期有关，等位基因Gln可能作为判断宫颈癌患者预后的分子标志物。

Relationship between the polymorphism of XRCC1-Arg399Gln and incidence risk of cervical cancer in the population of Guangdong

Objective To study the relationship between polymorphism of X - rag repair cross - complementing groups 1 （ XRCC1 ） - Arg399Gln and incidence risk of cervical cancer in the population of Guang Dong. Methods 162 women with cervical cancer and 183 age - matched normal women in Guangdong area were tested by the method of case - control study using Polymerase Chain Reaction - restriction fragment length polymorphism （PCR-RFLP）. The distribution frequency of XRCC1 -Arg399Gln genotype and its relationship with incidence risk of cervical cancer and clinico - pathological characteristic of patients were observed. Results The frequency distribution of Arg399Gln genotype（ Arg/Arg, Arg/Gln and Gln/Gln） was no statistic different between cervical cancer group and and control group. Polymorphism of Arg399Gln could not increase the incidence risk of cervical cancer. But Polymorphism of Arg399Gln was significantly ralated with clinic - stage of cervical cancer（ P 〈 0.05 ）. Distribution frequency of Arg/Gln and Gin/Gin genotype was significantly higher than Arg/Arg. Conclusion Arg399Gln polymorphism has no correlation with pathogenesis of cervical cancer. But it ralates with clinic - stage of cervical cancer. Allele gene Gin may be a molecular marker to estimate the prognosis of cervical cancer.