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| SMP1基因外显子多态性研究 | |
| 引用本文： | 周华友,杨贺才,张印则,李晓娟,于艳涛,刘持翔,陈蓉,王淑红,石文. SMP1基因外显子多态性研究[J]. 中国输血杂志, 2011, 24(5): 402-404 |
| 作者姓名： | 周华友 杨贺才 张印则 李晓娟 于艳涛 刘持翔 陈蓉 王淑红 石文 |
| 作者单位： | 1. 广州中医药大学第二附属医院,广东省中医院,输血科,广东广州510120 2. 深圳市血液中心 3. 西藏自治区血液中心 |
| 摘 要： | 目的建立SMP1基因外显子检测方法以探讨SMP1基因多态性。方法根据文献报道并参考Gene-Bank中的基因序列,设计聚合酶链式反应(PCR)扩增SMP1基因7个外显子的8对特异性引物,应用PCR分段扩增SMP1基因7个外显子,PCR产物纯化后直接测序,应用DNAMAN5.2.9序列分析软件分析序列。结果 SMP1基因第1~7外显子长度分别为137、106、113、68、93、60和1 703 bp。SMP1基因第1~6外显子未发现多态性位点,但该基因第7外显子存在1329T>C和1704G>A多态性。结论本研究成功建立SMP1基因外显子检测方法并检测到了SMP1基因第7外显子存在多态性位点。 |
| 关 键 词： | RH基因 SMP1基因 外显子 多态性 |
Study on the polymorphisms of SMP1 gene exons |
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| ZHOU Huayou,YANG Hecai,ZHANG Yinze,LI Xiaojuan,et al.. Study on the polymorphisms of SMP1 gene exons[J]. Chinese Journal of Blood Transfusion, 2011, 24(5): 402-404 | |
| Authors: | ZHOU Huayou YANG Hecai ZHANG Yinze LI Xiaojuan et al. |
| Affiliation: | ZHOU Huayou1,YANG Hecai1,ZHANG Yinze2,LI Xiaojuan3,et al.1.The Second Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510120,China,2.Shenzhen Blood Center,3.Tibet Blood Center |
| Abstract: | Objective To establish the detection method of SMP1 gene exons and investigate the polymorphism of SMP1 gene.Methods A total of 7 exons of SMP1 gene were amplified by polymerase chain reaction(PCR) with 8 pairs of specific primers designed according to reported sequences from literatures and GeneBank.Amplicons were sequenced after being purified and the sequences were analyzed with DNAMAN5.2.9 software.Results The lengths of exons 1~7 of SMP1 gene were 137,106,113,68,93,60 and 1 703 bp,respectively.Polymorp... |
| Keywords: | RH gene SMP1 gene Exon Polymorphism |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
