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| 吡那地尔对体外培养大鼠大脑皮层神经细胞缺氧缺糖损伤的保护作用 | |
| 引用本文： | 王华,麦根荣,陈宝芳,李涛,王卫民,潘同国,杨勇,谢集建.吡那地尔对体外培养大鼠大脑皮层神经细胞缺氧缺糖损伤的保护作用[J].武汉大学学报(医学版),2003,24(3):258-261,T001. |
| 作者姓名： | 王华 麦根荣 陈宝芳 李涛 王卫民 潘同国 杨勇 谢集建 |
| 作者单位： | 1. 武汉大学人民医院儿科,武汉,430060 2. 湖北省十堰市太和医院,儿科,十堰,442000 3. 湖北省十堰市太和医院,分子生物学实验室,十堰,442000 |
| 摘 要： | 目的 :探讨ATP敏感性K+ 通道 (KATP)开放剂吡那地尔 (pinacidil,Pin)对缺氧缺糖再复氧损伤大鼠大脑皮层神经细胞的保护作用。方法 :体外培养大鼠大脑皮层神经细胞 ,细胞培养至 10d ,建立神经细胞缺氧缺糖损伤模型 ,观察Pin及KATP阻断剂格列苯脲对缺氧缺糖不同时间 ,再复氧 2 4h后细胞死亡率、丙二醛 (MDA)含量、超氧化物歧化酶 (SOD)活力的影响。结果 :缺氧缺糖、再复氧后大鼠的神经细胞死亡率均显著升高、MDA生成增多、SOD的活力下降 ,Pin干预后 ,细胞死亡率下降、MDA生成减少、SOD的活力升高 ;格列苯脲能拮抗Pin这种保护作用。结论 :Pin对缺氧缺糖损伤神经细胞具有保护作用 ,并与拮抗氧自由基有关 |
| 关 键 词： | 吡那地尔 格列苯脲 ATP敏感性K+通道 细胞培养 |
Protective Effects of Pinacidil on Glucose-Oxygen Deprived Injury in Cultured Cortical Neurons in Rat |
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| Wang Hua,Mai Genrong,Chen Baofang,et al.Protective Effects of Pinacidil on Glucose-Oxygen Deprived Injury in Cultured Cortical Neurons in Rat[J].Medical Journal of Wuhan University,2003,24(3):258-261,T001. | |
| Authors: | Wang Hua Mai Genrong Chen Baofang |
| Affiliation: | Wang Hua,Mai Genrong,Chen Baofang,et al Department of Pediatric,Renmin Hospital,Wuhan University,Wuhan 430060,China |
| Abstract: | Objective: To investigate the protective effects of ATP sensitive potassium channel (K ATP ) opener Pinacidil on glucose oxygen deprived injury in cultured cortical neurons in newborn rats. Methods: When primary cultured neurons were exposed to glucose oxygen deprived condition for 4,8,16 h,reoxygen 24 h,the percentage of cell death and content of malonicaldehyde(MDA) and superoxide dimutase(SOD) were measured. Results: Pinacidil (10 -6 mol·L -1 ) reduced the percentage of cell death. It also prevented the elevation of MDA and increased the activities of SOD. K ATP agonist Glibenclamide could prevent the protective effects of Pinacidil. Conclusion: These results suggest that K ATP opener has neuronal protective effect,partly because it can suppress the generation of lipid peroxide and increase the activities of antioxidant enzymes. |
| Keywords: | pinacidil glibenclamide ATP sensitive potassium channel cell culture |
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