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苯并[a]芘对脊尾白虾(Exopalaemon carinicauda)肝脏生物标志物指标的影响
引用本文:李磊,蒋玫,沈新强,王云龙,吴庆元,牛俊翔,许高鹏. 苯并[a]芘对脊尾白虾(Exopalaemon carinicauda)肝脏生物标志物指标的影响[J]. 海洋环境科学, 2015, 34(4): 513-518. DOI: 10.13634/j.cnki.mes.2015.04.007
作者姓名:李磊  蒋玫  沈新强  王云龙  吴庆元  牛俊翔  许高鹏
作者单位:1.中国水产科学研究院东海水产研究所, 上海 200090;
摘    要:研究了脊尾白虾(Exopalaemon carinicauda)在对苯并[a]芘(benzo[a]pyrene,BaP)的富集(15 d)、释放(15 d)过程中肝脏的生物标志物(EROD、GST、SOD、CAT、GPx、MDA)的响应,结果显示:在富集阶段,第1 d低浓度实验组(0.05 g/L)和高浓度实验组(0.45 g/L)六种生物标志物即均显著受到诱导(P0.05),诱导程度与暴露浓度成正相关,而后不同种类生物标志物呈现差异性的变化趋势,EROD继续上升并在第10 d后达到稳定状态,GST继续上升并在第10 d后受到抑制下降,SOD、CAT、GPx均受到抑制逐渐下降,且均在15 d时低于对照组水平,MDA呈现持续上升的趋势,直接反映了肝脏的氧化损伤程度。在释放阶段,低浓度实验组各生物标志物的恢复速度较高浓度实验组要快,且均能恢复到接近对照组水平(P0.05),高浓度实验组各生物标志物则均未能恢复到对照组水平(P0.05)。实验结果表明,脊尾白虾肝脏具有一定恢复能力,各生物标志物对BaP暴露的敏感性具有一定差异,BaP暴露的浓度及时间是影响各生物标志物响应变化的主要因素。

关 键 词:苯并[a]芘   脊尾白虾   肝脏   生物标志物
收稿时间:2014-03-20

Effects of benzo[a]pyrene exposure on biomarkers in Exopalaemon carinicauda liver
LI Lei,JIANG Mei,SHEN Xin-qiang,WANG Yun-long,WU Qing-yuan,NIU Jun-xiang,XU Gao-peng. Effects of benzo[a]pyrene exposure on biomarkers in Exopalaemon carinicauda liver[J]. Marine Environmental Science, 2015, 34(4): 513-518. DOI: 10.13634/j.cnki.mes.2015.04.007
Authors:LI Lei  JIANG Mei  SHEN Xin-qiang  WANG Yun-long  WU Qing-yuan  NIU Jun-xiang  XU Gao-peng
Affiliation:1.East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China;
Abstract:Benzo[a]pyrene (BaP) is one of the most important pollutants in the marine environment.Effects of biomarkers which including EROD,GST,SOD,CAT,GPx,MDA in the liver of Exopalaemon carinicauda with BaP exposure and elimination were detected in the exposure period (fifteen days) and elimination period (fifteen days).The outcomes indicated that the activities of six biomarkers were significantly induced by BaP in the first day (P0.05) and exhibited a direct correlation with the concentration of BaP,then indicated a different change trend between six biomarkers.The activities of EROD continued to grow until it hit a steady state in ten days,the activities of GST continued to grow and then went down slightly in ten days,the activities of three antioxidant enzymes (SOD,CAT,GPx) gradually declined and lower than in the control group in fifteen days,the concentration of MDA continued to grow during the exposure period,established as a direct biomarker reflecting the oxidant stress.In the evacuation period,the lower concentration groups resumed quickly than the higher concentration groups,all biomarkers could resumed to the command level (P0.05),while the high concentration,in contrast,could not resumed to the command level (P0.05).The results of this experiment indicated that the E.Carinicauda possessed resume capacity,there were a certain degree difference between sensitivity of all bookmakers,factors including BaP concentration and exposure time had an important impression on six biomarkers activities.
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