Resolution and reconstitution of complex II (succinate-ubiquinone reductase) by salts |
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| Authors: | K A Davis Y Hatefi |
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| Affiliation: | 1. Department of Housing Environmental Design and Research Institute of Human Ecology, College of Human Ecology, Jeonbuk National University, Jeonju, Republic of Korea;2. Department of Polymer-Nano Science and Technology, Jeonbuk National University, Jeonju, Republic of Korea;1. Institute of Biotechnology, Shiraz University, Shiraz, 71441-65186, Islamic Republic of Iran;2. Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran;1. Department of Environmental Science and Engineering, Hubei Environmental Remediation Material Engineering Technology Research Center, Wuhan University, Wuhan 430079, China;2. Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, Department of Environmental Engineering, Hubei Normal University, Huangshi 435002, China;1. Grupo de Eletroquímica e Novos Materiais (GENMAT), Departamento de Química, Universidade Federal de Mato Grosso (UFMT), Cuiabá, MT CEP 78060-900, Brazil |
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| Abstract: | The effects of representative chaotropic ions (NO3−, ClO4−, Cl3CCOO−) on the resolution, and antichaotropic ions (SO4−2, HPO4−, F−) on the reconstitution of a water-insoluble multiprotein-lipid complex (succinate-ubiquinone reductase or complex II) have been studied. Succinate dehydrogenase appears to be bound to the components of the complex mainly by hydrophobic attractions. This association is considerably stronger when the complex is suspended in D2O rather than in H2O. The resolution of complex II with respect to succinate dehydrogenase is an equilibrium process. The equilibrium can be shifted in the direction of resolution by chaotropic salts, and in the direction of reconstitution of complex II either by removal of the added chaotrope or by addition of an antichaotropic salt. The chaotrope-induced resolution of complex II has a small but significant temperature dependence. As might be expected, the equilibrium of a partially resolved complex II system can be shifted in either direction by altering the temperature of the medium. Similar to complexes I and III, the reduced form of complex II appears to be more stable to resolution than its oxidized form. |
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