Methods of Investigation of the Metabolism of Anaerobic Microorganisms

At present the development together with an intensification of anaerobic processes calls for methods of observation and control. It is very important to know intermediate metabolites of anaerobic microorganisms and the activity of methanogenic ones. Presented analytical methods are GLC analyses of lower fatty acids, alcohols, aldehydes and ketones and components of biogas. The chromatography using steam as a mobile phase is also mentioned. The method of determining the fluorescent coenzyme F₄₂₀ was tested and modified. This coenzyme is specific for methanogenic microorganisms and is closely linked with their activity. The determination of the total activity of the hydrogenase system of anaerobic microorganisms by means of the rate of the consumption and evolution of molecular hydrogen was also presented. The applicability and effectivenes of these methods were verified on natural samples. All the methods mentioned above give satisfactory results with available equipment, materials and techniques.