| ~(125)I粒子抑制乳腺癌细胞bFGF表达的实验研究 |
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| 引用本文： | 张万福,王明春,罗开元,李波,杨嵘,章治平,李晓刚,褚焱. ~(125)I粒子抑制乳腺癌细胞bFGF表达的实验研究[J]. 中国现代普通外科进展, 2010, 0(6) |
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| 作者姓名： | 张万福 王明春 罗开元 李波 杨嵘 章治平 李晓刚 褚焱 |
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| 作者单位： | 昆明医学院第四附属医院普外科; |
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| 基金项目： | 国家自然科学基金(30660183) |
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| 摘 要： | 目的:探讨125I粒子对乳腺癌细胞中碱性成纤维细胞因子(bFGF)表达的影响及其在抑制肿瘤细胞增殖方面的作用机制。方法:建立人乳腺癌细胞株MCF-7裸鼠皮下移植瘤模型,根据处死时间,随机分为8组,包括D7、D14、D21、D28组(对照组)及S7、S14、S21、S28组(实验组),每组6只,对照组植入空白粒子(0 mCi),实验组植入125I粒子(0.4 mCi)。观察粒子植入后瘤体生长情况,粒子植入后第7、14、21、28天分别处死各组荷瘤小鼠。半定量RT-PCR、免疫组织化学染色方法检测bFGF mRNA及其蛋白的表达。结果:植入125I粒子后,实验组肿瘤生长缓慢,肿瘤抑制率为53.27%。粒子植入第28天,与对照组相比,实验组肿瘤组织的bFGF mRNA及蛋白表达明显降低,差异有统计学意义(χ2=45.788,P<0.01)。结论:125I粒子抑制bFGF mRNA和蛋白的表达是其治疗乳腺癌增殖的分子生物学机制之一。
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| 关 键 词： | 碘 乳腺肿瘤 成纤维细胞生长因子2 小鼠 裸 |
Experiment research on ~(125)I interstitial rachytherapy inhabiting the express of bFGF in breast cancer cell |
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| ZHANG Wan-fu,WANG Ming-chun,LUO Kai-yuan,LI Bo,YANG yong,ZHANG Zhi-ping,LI Xiao-gang,CHU Yan. Experiment research on ~(125)I interstitial rachytherapy inhabiting the express of bFGF in breast cancer cell[J]. Chinese Journal of Current Advances in General Surgery, 2010, 0(6) |
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| Authors: | ZHANG Wan-fu WANG Ming-chun LUO Kai-yuan LI Bo YANG yong ZHANG Zhi-ping LI Xiao-gang CHU Yan |
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| Affiliation: | ZHANG Wan-fu,WANG Ming-chun,LUO Kai-yuan,LI Bo,YANG yong,ZHANG Zhi-ping,LI Xiao-gang,CHU Yan Department of General Surgery,the Fourth Affiliated Hospital of Kunming Medical College(Kunming 650021,China) |
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| Abstract: | Objective: To investigate the mechanism of 125I refraining the growth of tumors cell by hindering the expression of basic firoblast growth factor(bFGF)in breast cancer.Methods: The animal models of the breast cancer cell(MCF-7) were established by subcutaneously injecting the tumor cells into nude mice.The mice were divided into eight groups randomly and therewere six nude mice in each group(the control groups contained D7,D14,D21,D28 groups;theexperiment groups contained S7,S14,S21,S28 groups).No-load seed... |
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| Keywords: | Iodine.Breast neoplasms.Firoblast growth factor 2.Mice nude |
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