野生大豆皂苷的提取与薄层色谱分析

皂苷具有多种药理作用,对野生大豆中各类皂苷的定量分析是研究野生大豆皂苷药理作用的基础.利用索氏提取器以甲醇热回流提取野生大豆中的皂苷,结合理化反应进行鉴别,以齐墩果酸作为对照品进行薄层层析,通过薄层扫描测定皂苷含量.结果表明:野生大豆皂苷1～5μg范围内线性关系良好,平均回收率为98.98％,RSD为0.41％,薄层层...

Extraction and Thin Layer Chromatography of Saponins from Wild Soybean

Saponins have many pharmacological actions.The determination of wild soybean saponins contents is the basis of the pharmacological experiments.In this experiment,wild soybean saponins were extracted by Soxhlet extractor with hot circulatory methanol,identified by physical-chemical reactions and the thin layer chromatography(TLC)with the oleanic acid taken as reference standard,and then the TLC scanner was used to determine the content of the saponins.The linear range of wild soybean saponins was from 1 to 5...