普洱熟茶不同溶剂萃取层中儿茶素及黄酮醇类化合物差异的研究

目的 探索普洱熟茶在不同溶剂(乙酸乙酯和正丁醇)萃取中儿茶素和黄酮醇类化合物的组成及含量的差异,构建一种有效提取普洱熟茶中儿茶素及黄酮醇类物质的方法.方法 以普洱熟茶(2008年生产)的水提物为研究对象,分别用乙酸乙酯和正丁醇溶剂萃取的萃取层为分析供试样,采用高效液相色谱法(high performance liqui...

Study on catechins and flavonols materials differences in different solvent extracts of Pu-erh ripened tea

Objective To explore the composition and content differences of catechins and flavonols in different solvents (ethyl acetate and n-butanol) of Pu-erh ripened tea, and establish an effective extraction method of catechins and flavonols in Pu-erh ripened tea. Methods The water extract of Pu-erh ripened tea (2008) was used as the research object. The extraction layer was extracted with ethyl acetate and n-butanol, respectively. The contents of 5 catechins and 4 flavonols in the tea samples were determined by high performance liquid chromatography (HPLC). Results +Catechin, epicatechin, epicatechin gallate, epigallocatechin gallate, myricetin, quercetin and kaempferol were mainly enriched in the ethyl acetate extract. Rutin and epigallocatechin were enriched in the n-butanol layer and the residual layer, respectively. Conclusion A simple and effective extraction method for the analysis and detection of catechins and flavonols in Pu-erh ripened tea is established, which provides a reference for further analysis of the chemical constituents of Pu-erh ripened tea.