DMSO respiration by the anaerobic rumen bacterium Wolinella succinogenes |
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Authors: | Jürgen Lorenzen Swantje Steinwachs Gottfried Unden |
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Affiliation: | (1) Institut für Biochemie, Heinrich-Heine-Universität Düsseldorf, Universitätsstrasse 1, D-40225 Düsseldorf, Germany;(2) Institut für Mikrobiologie und Weinforschung, Johannes Gutenberg Universität, Becherweg 15, D-55099 Mainz, Germany |
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Abstract: | The anaerobic rumen bacterium Wolinella succinogenes was able to grow by respiration with dimethylsulphoxide (DMSO) as electron acceptor and formate or H2 as electron donors. The growth yield amounted to 6.7 g and 6.4 g dry cells/mol DMSO with formate or H2 as the donors, respectively. This suggested an ATP yield of about 0.7 mol ATP/mol DMSO. Cell homogenates and the membrane fraction contained DMSO reductase activity with a high Km (43 mM) for DMSO. The electron transport from H2 to DMSO in the membranes was inhibited by 2-(heptyl)-4-hydroxyquinoline N-oxide, indicating the participation of menaquinone. Formation of DMSO reductase activity occurred only during growth on DMSO, presence of other electron acceptors (fumarate, nitrate, nitrite, N2O, and sulphur) repressed the DMSO reductase activity. DMSO can therefore be used by W. succinogenes as an acceptor for phosphorylative electron transport, but other electron acceptors are used preferentially.Abbreviations DMN 2,3-Dimethyl-1,4-naphthoquinone - DMNH2 Reduced DMN - DMS Dimethylsulphide (CH3)2S - DMSO Dimethylsulphoxide (CH3)2SO - HQNO 2-(Heptyl)-4-hydroxyquinoline-N-oxide - TMAO Trimethylamine-N-oxide - Ys Growth yield for substrate S |
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Keywords: | DMSO respiration DMS production Anaerobic respiration Anaerobic regulation Wolinella succinogens |
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