| 海洋低温BS041208菌株选育及发酵培养基的研究(Ⅱ) |
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| 引用本文： | 迟乃玉,张庆芳,窦少华,唐乾,袁玉莲.海洋低温BS041208菌株选育及发酵培养基的研究(Ⅱ)[J].食品与发酵工业,2006,32(4):35-37. |
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| 作者姓名： | 迟乃玉 张庆芳 窦少华 唐乾 袁玉莲 |
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| 作者单位： | 1. 大连大学生物工程学院,大连,116622;大连大学生物有机化学重点实验室,大连,116622
2. 大连大学生物工程学院,大连,116622 |
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| 摘 要： | 建立了海洋低温蛋白酶菌株(Bacillussubtilis简写为BS041208)发酵最适pH值、温度、时间、接种量、通气量分别为5.0、14℃、48h、8%、150mL;在最适发酵条件下,BS041208菌株50L发酵罐中低温蛋白酶活性为1371.8U/mg。
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| 关 键 词： | Bacillussubtilis 低温蛋白酶 发酵条件 |
| 收稿时间： | 02 24 2006 12:00AM |
| 修稿时间： | 2006年2月24日 |
The Screening of the Marine Low Temperature Acid Protease Strain from Bacillus subtilis and Its Optimal Fermentation Medium( Ⅱ ) |
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| Chi Naiyu,Zhang Qingfang,Dou Shaohua,Tang Qian,Yuan Yulian.The Screening of the Marine Low Temperature Acid Protease Strain from Bacillus subtilis and Its Optimal Fermentation Medium( Ⅱ )[J].Food and Fermentation Industries,2006,32(4):35-37. |
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| Authors: | Chi Naiyu Zhang Qingfang Dou Shaohua Tang Qian Yuan Yulian |
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| Affiliation: | 1.College of Bioengineering, Dalian University, Dalian 116622, China ; 2.Key Laboratory of Bio-organic Chemistry, Dalian University, Dalian 116622, China |
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| Abstract: | The fermentation conditions of high Marine low temperature acid protease producing strain BS041208 were determined in this paper. The optimum conditions of producing marine low temperature acid protease were: initial pH 5.0,temperature 14℃, culture time 48 h, inoculum size 8%,aeration 150 mL. Under the optimum conditions:the marine low temperature acid protease yield of BS041208 up to 1 371.8 U/mg in 50 L fermentor. |
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| Keywords: | Bacillus subtilis |
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