山楂叶总黄酮对血管性痴呆大鼠学习记忆及海马AChE, ChAT活性的影响

目的:探讨山楂叶总黄酮(total flavone of Hawthorn leaf,TFHL)对血管性痴呆大鼠空间学习记忆障碍的治疗作用及机制。方法:健康雄性SD大鼠64只,随机分为假手术组(sham control group)、模型组(model group)、TFHL 70,140 mg·kg-1及银杏叶片组(3.5 mg·kg-1),每组16只。采用永久性双侧颈总动脉结扎法制备血管性痴呆大鼠模型,TFHL 70,140 mg·kg-1组连续给药36 d。采用Morris水迷宫评价大鼠的空间学习学习记忆能力,分光光度比色法检测大鼠海马乙酰胆碱酯酶(AChE)和乙酰胆碱转移酶(ChAT)活性。结果:与假手术组比较,血管性痴呆模型组大鼠学习记忆能力显著降低,大鼠逃避潜伏期明显延长(P<0.05),穿越平台区次数显著减少(P<0.05),平台象限游泳距离百分比显著降低(P<0.05);ChAT活性明显降低(P<0.05),AChE活性显著增强(P<0.05)。TFHL及银杏叶片能改善血管性痴呆大鼠学习记忆能力,缩短大鼠逃避潜伏期(P<0.05),增加大鼠穿越平台次数及平台象限游泳距离百分比(P<0.05),增强ChAT活性(P<0.05),降低AChE活性(P<0.05)。结论:TFHL可通过提高海马ChAT活性、降低AChE活性改善血管性痴呆大鼠海马胆碱能神经系统的功能,从而改善血管性痴呆大鼠的学习记忆功能。

Effect of Total Flavone of Hawthorn Leaves on Activity of Learning and Memory and AChE and ChAT in Brian of Vascular Dementia Rat Model

Objective: To investigate the total flavone of Hawthorn Leaves(TFHL) on the therapeutic effect and mechanism of spatial learning and memory impairment of the vascular dementia rat. Method: Sixty-four SD male rats were randomly divided into sham control group, model group, TFHL 70, 140 mg·kg^-1 group, Sixteen in each group. Permanent bilateral carotid artery ligation was used to prepare vascular dementia model.TFHL 70, 140 mg·kg^-1 group were given for consecutive 36 days. Morris water maze was used for evaluation of learning and memory abilities of rats. Colorimetric detection was used to detect hippocampus acetyl cholinesterase (AChE), choline acetyltransferase(ChAT) content. Result: Compared with the sham control group, in vascular dementia model group learning and memory capacity significantly reduced, escape incubation period extended significantly (P<0.05), the number of crossing the platform area significantly reduced (P<0.05), significantly reduced percentage of target quadrant distance(P<0.05);ChAT activity was significantly decreased (P<0.05), AChE activity was significantly increased (P<0.05). TFHL and Yinxingye pian could improve learning and memory ability, shorten the the rats escape latency (P<0.05),increase the number of across the platform and the platform quadrant percentage of swimming distance (P<0.05), enhance ChAT activity (P<0.05), reduce AChE activity (P<0.05). Conclusion: TFHL can improve learning and memory impairment of vascular dementia rat, may enhance ChAT activity, reduce the activity of AChE, increase the acetylcholine content of brain tissue, restore cholinergic nerve system function related to chronic cerebral ischemia in rats.