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| 贵州和浙江产吴茱萸指纹图谱分析及含量测定 | |
| 引用本文： | 张红梅,李国,宋龙,赵志礼,王长虹,王峥涛. 贵州和浙江产吴茱萸指纹图谱分析及含量测定[J]. 中国实验方剂学杂志, 2014, 20(17): 69-73 |
| 作者姓名： | 张红梅 李国 宋龙 赵志礼 王长虹 王峥涛 |
| 作者单位： | 1. 上海中医药大学中药学院,中药研究所,中药标准化教育部重点实验室,上海201203 2. 浙江星光农业开发有限公司,浙江平阳,325400 3. 上海中医药大学中药学院,中药研究所,中药标准化教育部重点实验室,上海201203;上海中药标准化研究中心,上海201210 |
| 基金项目： | 上海市卫生局青年科研项目（20114y022） |
| 摘 要： | 目的:应用UPLC-UV指纹图谱方法,结合吴茱萸中有效成分柠檬苦素、吴茱萸碱和吴茱萸次碱的含量测定,评价不同品种及产地的吴茱萸药材质量,为控制市场上吴茱萸药材质量提供依据。方法:采用ACQUITY UPLC HSS T3色谱柱(2.1 mm×100 mm,1.8μm),流动相乙腈-水梯度洗脱,流速0.5 mL·min-1,检测波长210 nm,柱温25℃。收集了24批不同产地不同种吴茱萸样品进行指纹图谱分析,结合主成分分析及有效成分含量测定,对吴茱萸药材整体质量进行评价。结果:建立了吴茱萸UPLC-UV指纹图谱,标定了9个共有峰。指纹图谱相似度评价结果显示贵州产吴茱萸质量较稳定、统一,浙江产吴茱萸质量稍不统一。主成分分析结果表明,吴茱萸药材不同种之间成分上存在差异性。产地对吴茱萸药材中柠檬苦素和生物碱含量有影响。结论:指纹图谱结合有效成分含量测定的方法可用于吴茱萸药材的整体质量评价,对吴茱萸药材的品种鉴定及质量控制具有指导意义。 |
| 关 键 词： | 吴茱萸 柠檬苦素 吴茱萸碱 吴茱萸次碱 指纹图谱 主成分分析 超高效液相色谱 |
| 收稿时间： | 2013-12-01 |
Fingerprint and Content Determination of Euodiae Fructus Collected in Guizhou and Zhejiang |
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| ZHANG Hong-mei,LI Guo,SONG Long,ZHAO Zhi-li,WANG Chang-hong and WANG Zheng-tao. Fingerprint and Content Determination of Euodiae Fructus Collected in Guizhou and Zhejiang[J]. China Journal of Experimental Traditional Medical Formulae, 2014, 20(17): 69-73 | |
| Authors: | ZHANG Hong-mei LI Guo SONG Long ZHAO Zhi-li WANG Chang-hong WANG Zheng-tao |
| Affiliation: | The Key Laboratory for Standardization of Chinese Medicines, Ministry of Education, Institute of Chinese Materia Medica, College of Traditional Chinese Medicine(TCM), Shanghai University of TCM, Shanghai 201203, China;Zhejiang Starlight Agricultural Development Co. Ltd, Pingyang 325400, China;The Key Laboratory for Standardization of Chinese Medicines, Ministry of Education, Institute of Chinese Materia Medica, College of Traditional Chinese Medicine(TCM), Shanghai University of TCM, Shanghai 201203, China;The Key Laboratory for Standardization of Chinese Medicines, Ministry of Education, Institute of Chinese Materia Medica, College of Traditional Chinese Medicine(TCM), Shanghai University of TCM, Shanghai 201203, China;The Key Laboratory for Standardization of Chinese Medicines, Ministry of Education, Institute of Chinese Materia Medica, College of Traditional Chinese Medicine(TCM), Shanghai University of TCM, Shanghai 201203, China;Shanghai R&D Centre for Standardization of TCM, Shanghai 201210, China;The Key Laboratory for Standardization of Chinese Medicines, Ministry of Education, Institute of Chinese Materia Medica, College of Traditional Chinese Medicine(TCM), Shanghai University of TCM, Shanghai 201203, China;Shanghai R&D Centre for Standardization of TCM, Shanghai 201210, China |
| Abstract: | |
| Keywords: | Euodiae Fructus limonin evodiamine rutaecarpine fingerprint principal component analysis UPLC |
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