Bcl—2在醛固酮腺瘤模型大鼠主动脉中的表达

目的：观察醛固酮对主动脉Bcl-2表达的影响。方法：32只SD大鼠随机均分为4组：①空白溶刹口为对照（60％丙二醇＋10％乙醇＋30％双蒸水）；②醛固酮腺瘤：泵入醛固酮（1／,g／h）；③醛固酮腺瘤＋依普利目组：依普利酮（100mg·kg-1·d-1）；④醛固酮腺瘤＋肼苯哒嗪组：肼苯哒嗪（25mg·kg-1·d-1）。渗透泵内分别注入醛固酮或空白溶剂，然后将其埋植于大鼠背部皮下。8周后，免疫组织化学和免疫印迹检测主动脉Bcl的表达。结果：与对照组相比，腺瘤组大鼠主动脉Bcl—2表达显著减少（P〈0．05）；依普利酮能够拮抗醛同酮对Bcl—2表达的抑制作用（P〈20．05）。结论：醛固酮通过抑制Bcl—2表达，调节细胞凋亡状态，可能足醛固酮诱导主动脉重构的机制之一。

Bcl-2 Expression in the Aortas of Model Rats with Aldosterone-Producing Adenomas

Objective:To observe the effect of aldosterone on Bcl-2 expression in the aorta.Methods:Thirty-two male rats were randomly divided into 4 groups;vehicle(control),aldosterone-producing adenoma,aldosterone -producing adenoma plus eplerenone or hydralazine.They were then implanted with an osmotic mini-pump that infused either aldosterone or the vehicle.After 8 weeks.Bcl-2 was examined by immunohistochemistry and Western blotting.Results:Compared with controls,the model rats with aldosterone-producing adenoma exhibited decreased aortic expression of Bcl-2.This effect of aldosterone was significantly reversed after co-treatment with eplerenone but not with hydralazine.Conclusions:This study’s findings suggest that aldosterone directly suppresses aortic expression of Bcl-2 that may play an important role in aldosterone-induced aortic remodeling.