| 镧掺杂二氧化钛/沸石微柱在线预富集-分光光度法测定溶菌酶含量 |
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| 引用本文： | 田苗苗,苏日艳,贾琼,鲍长利,权新军.镧掺杂二氧化钛/沸石微柱在线预富集-分光光度法测定溶菌酶含量[J].分析化学,2011,39(1):103-106. |
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| 作者姓名： | 田苗苗 苏日艳 贾琼 鲍长利 权新军 |
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| 作者单位： | 吉林大学化学学院; |
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| 基金项目： | 中国科学院长春应用化学研究所稀土资源利用国家重点实验室开放基金(2008)项目 |
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| 摘 要： | 以镧掺杂二氧化钛/沸石吸附剂微柱为萃取装置,建立了溶菌酶的分光光度检测法.考察了实验条件对萃取效率的影响.以甲醇为洗脱液,流速2.0 mL/min;样品的pH=5.0,流速1.2 mL/min,体积1.2 mL;检测波长280 nm.对蛋清、蜂蜜及葡萄酒中溶菌酶的含量进行了测定,日内与日间精密度分别为2.1%和2.8%...
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| 关 键 词： | 镧掺杂二氧化钛/沸石 微柱 分光光度法 溶菌酶 |
Spectrophotometric Determination of Lysozyme by On-line Preconcentration with A Microcolumn Containing La3+-TiO2-Zeolite |
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| TIAN Miao-Miao,SU Ri-Yan,JIA Qiong,BAO Chang-Li,QUAN Xin-Jun.Spectrophotometric Determination of Lysozyme by On-line Preconcentration with A Microcolumn Containing La3+-TiO2-Zeolite[J].Chinese Journal of Analytical Chemistry,2011,39(1):103-106. |
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| Authors: | TIAN Miao-Miao SU Ri-Yan JIA Qiong BAO Chang-Li QUAN Xin-Jun |
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| Affiliation: | TIAN Miao-Miao,SU Ri-Yan,JIA Qiong*,BAO Chang-Li,QUAN Xin-Jun(College of Chemistry,Jilin University,Changchun 130022) |
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| Abstract: | A spectrophotometric method for the determination of lysozyme was established based on the on-line preconcentration with a La3 +-TiO2-zeolite microcolumn.Effects of operation parameters on the extraction efficiency were optimized as follows:methanol as the eluent with a flow rate of 2.0 mL/min,sample pH of 5.0,sample flow rate of 1.2 mL/min,sample volume of 1.2 mL,and detection wavelength of 280 nm.The proposed method was employed for the determination of lysozyme in egg white,honey,and wine samples.The rel... |
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