生脉散对急性肝衰竭大鼠内毒素诱导细胞因子的影响

目的]探讨生脉散对急性肝衰竭大鼠内毒素（LPS）诱导细胞因子水平的影响。方法]采用胁氨基半乳糖腹腔注射法制作急性肝衰竭大鼠模型。予生脉散灌胃2h及8h后，检测血清LPS与细胞因子水平。结果]D-氨基半乳糖能明显增加大鼠血清白细胞介素6（IL-6）、细胞间黏附分子1（ICAM-1）和IL-1β水平（P〈0．01），随着D-氨基半乳糖作用时间延长的趋势明显减弱，但对血清LPS、肿瘤坏死因子α（TNF-α）水平无明显影响（P〉0．05）；生脉散能显著降低D氨基半乳糖肝衰竭大鼠血清IL-6、ICAM-1和IL-1β水平（P〈0．01，〈O．05）。LPS攻击2h和8h后，能明显诱导D-氨基半乳糖大鼠血清LPS、TNF-α、IL-6、ICAM-1和IL-1β水平增加（P〈0．01）；除在8h对TNF-α无影响外，生脉散能明显减轻LPS攻击D-氨基半乳糖大鼠后对血清LPS、TNF-α、IL-6、ICAM-1和IL-1β水平的影响（P〈0．01，〈O．05）。结论]SD大鼠在急性肝衰竭状态下，存在严重LPS血症，并引起细胞因子水平变化的炎症级联反应，生脉散可通过调节细胞及炎症因子水平起到治疗作用。

Effects of Sheng Mai San on the serum level of cytokines induced by lipopolysaccharide in acute liver failure rats

Objective]To study the effects of Sheng Mai San on the levels of cytokines induced by lipopolysaccharide in acute liver failure rats. Methods]The models of acute liver failure were made by injecting D-galactoasmine in the abdomen of SD rats, 2 and 8 hours after treating with LPS and Sheng Mai San,the serum level of lipopolysaccharide and cytokines were detected. Resuits]The serum level of IL-6, ICAM-1 and IL-1β was increased by D-gal （P〈0. 01）. Prolonging the time,the effects of D-Gal reduced significantly, but there was no effect on the serum level of LPS and TNF-α in rats （P〉0.05）. ShengMaiSan could significantly reduce the serum level of IL- 6,ICAM-1 and IL-1β in rats with acute liver failure induced by D-Gal （P〈0. 01,P〈0. 05）. Two and eight hours after treating with I.PS,the serum level of LPS,TNF-α, IL-6, ICAM-1 and IL-1β markedly increased （P〈0. 01）. Except that in the time point of 8h there was no influence on TNF-α（P〉0. 05）,ShengMaiSan could reduce the serum level of LPS, TNF-α,IL-6, ICAM-1 and IL-1β in rats with acute liver failure（P〈0.01）. Conclusion]SD rats in the state of acute liver failure,existing serious serum endotoxin, and D-Gal can induce the increasing of cytokines, Sheng- MaiSan can improve it through regulating the level of cytokines.