| LC-MS~n法研究曲马多在大鼠尿中的代谢产物 |
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| 引用本文： | Lu H,Wu D,Yan J,Zeng Q,Wu YP,Sun L. LC-MS~n法研究曲马多在大鼠尿中的代谢产物[J]. 药学学报, 2011, 46(8): 968-975 |
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| 作者姓名： | Lu H Wu D Yan J Zeng Q Wu YP Sun L |
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| 作者单位： | 沈阳药科大学药学院;辽宁省公安厅刑事科学技术研究所; |
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| 摘 要： | 研究曲马多在大鼠尿中的主要代谢产物。健康大鼠腹腔注射盐酸曲马多(剂量为9.0 mg.kg-1),收集0～36 h不同时间段的尿样,经固相萃取法预处理后,直接采用LC-MSn法对曲马多和代谢产物进行分析。通过比较空白尿样和给药后尿样的总离子流色谱图和选择离子监测色谱图,与3个代谢产物对照品比较,并分析各个色谱峰的色谱保留时间、准分子离子和多级碎片离子,在大鼠尿样中共检测到13种代谢产物,包括9种I相代谢产物,4种II相代谢产物,其中1种为首次在生物体内发现的曲马多代谢产物,证明曲马多在大鼠体内的主要代谢途径为O-去甲基化、N-去甲基化、羟基化、N-氧化和葡糖醛酸化反应。本文所建立的LC-MSn方法可进一步研究曲马多在其他动物和人体内的代谢产物。
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| 关 键 词： | LC-MSn 曲马多 代谢产物 |
Analysis of primary metabolites of tramadol in rat urine by LC-MS(n |
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| Lu Hui,Wu Di,Yan Jing,Zeng Qin,Wu Yan-Ping,Sun Lu. Analysis of primary metabolites of tramadol in rat urine by LC-MS(n[J]. Acta pharmaceutica Sinica, 2011, 46(8): 968-975 |
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| Authors: | Lu Hui Wu Di Yan Jing Zeng Qin Wu Yan-Ping Sun Lu |
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| Affiliation: | LU Hui1,WU Di2,YAN Jing1,ZENG Qin1,WU Yan-ping1,SUN Lu1(1.School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China,2.Criminal Science and Technology Institute,Department of Public Security of Liaoning Province,Shenyang 110032,China) |
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| Abstract: | Tramadol and its metabolites in rat urine were identified by LC-MSn.Rat urine samples of 0-36 h were collected after ip 9.0 mg-kg-1 tramadol,then the samples were enriched and purified through solid-phase extraction cartridge.Purified samples were analyzed by LC-MSn.Possible metabolites were discovered by comparing the full scan and SIM chromatograms of the test samples with the corresponding blanks and analyzing the retention time,quasi-molecular ion and fragment ion of all chromatograms.Nine phase I metab... |
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| Keywords: | LC-MSn tramadol metabolite |
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