Wistar大鼠脑和脊髓微血管周细胞的分离、培养、鉴定及其功能差异的比较

探讨Wistar大鼠脑微血管周细胞（brain microvascular pericyte,BMP）和脊髓微血管周细胞（spinal cord microvascular pericyte,SCMP）之间的差异。运用超高速离心法获取脑和脊髓微血管,再用周细胞培养基培养,使周细胞爬出,然后用NG2和PDGFRβ鉴定周细胞,用鬼笔环肽着染F-actin,用流式细胞仪测定细胞周期,用免疫印记分析实验测定周细胞功能蛋白。结果表明：两种周细胞的形态有明显差异,BMP表达的F-actin显著多于SCMP,两种周细胞的细胞周期无显著差异,BMP相比于SCMP表达更多量的α-SMA、NG2和PDGFRβ。更多的了解两种周细胞的异同点,为研究其在中枢神经系统生理和病理状态下的作用奠定基础。

Isolated,Cultivated,Identified and Comparison of Brain and Spinal Cord Microvascular Pericytes from Wista Rats

To compare the different characteristics of brain microvascular pericytes（ BMP） and spinal cord microvacularpericytes（ SCMP）,they were isolated,cultured and identified. High-speed centrifugal method was used to obtain the microvassels from brain and spinal cord. Then the microvessels were cultivated in the pericyte-specific medium. Pericyte was isolated from the microvessels. NG2 and PDGFRβ were used to identify the pericytes. FITC-labeled phalloidin was used to stain F-actin. The cell cycles of BMP and SCMP were detected by flow cytometry. Western blot assay was applied to analyze the functional proteins of pericytes. The results indicated that BMPs possessed bigger cell nucleus and more unfolded cytoplasm than SCMPs. BMPs expressed much more F-actin than SCMPs（ P〈0. 01）. There was no significant difference between the two types of pericytes in cell cycle. BMP expressed more α-SMA、NG2 and PDGFRβ than SCMPs. These distinguishing features may reflect the more widespread differences between the BBB and BSCB which directly impact pathophysiological processes in various major diseases.