胶束电动色谱在线推扫技术测定血液中环丙沙星含量

采用在线推扫技术建立了胶束电动色谱法测定血液中痕量环丙沙星的方法.考察了背景缓冲溶液pH值、SDS浓度、进样时间对环丙沙星富集效果的影响.结果表明:在最佳分析条件下,环丙沙星的检测灵敏度可提高200倍.本方法减少了样品处理的繁琐过程,缩短了分析周期,弥补了毛细管电泳测定痕量组分的不足,为毛细管电泳的体内药物分析及药代动力学研究、临床痕量药物检测提出了新的途径.

Determination of Ciprofloxacin in Animal Plasma by MEKC and On-line Sweeping Technique

In this work, an on-line sweeping technique was developed to determine ciprofloxacin in animal plasma by MEKC. The effect of SDS concentration, pH value, running voltage and injection time on the concentration folds were investigated. The result shows that under the optimum conditions, more than 200 fold enrichment of ciprofloxacin was obtained with good linear relation within the range of ciprofloxacin plasma concentration of 0.05～10 mg/L(r=0.999).