全自动氨基酸分析仪法测定不同年份黄酒中游离氨基酸的含量

目的建立采用全自动氨基酸分析仪测定黄酒中游离氨基酸的含量的方法,并测定不同年份的黄酒中游离氨基酸含量。方法黄酒经氮气吹干复溶后,过膜,使用全自动氨基酸分析仪进行测定。结果方法检出限达到0.01 mg/100 m L,回收率达到90.1%~101.2%,均符合检测要求。测定结果显示,黄酒中含有脯氨酸、亮氨酸等多种氨基酸,具有较高的营养价值,且不同酒龄的黄酒中氨基酸的总含量不同,各氨基酸含量随年份的不同也会发生变化。结论该方法前处理简单、快速,测定结果准确、可靠,能够满足黄酒中氨基酸的日常检测工作,适用于大量样品的定性定量分析。

Determination of amino acids content in yellow rice wine of different years with automatic amino acid analyzer

Objective To develop a method for the determination of free amino acids in yellow rice wine with automatic amino acid analyzer, and determine the free amino acid srice wine with different ages. Methods The yellow rice wine were rehydrated after dried by nitrogen, and then determined by automatic amino acid analyzer. Results The detection limit of the method was 0.01mg/100 mL, and the recoveries could range from 90.1%~101.2%, meeting the requirements of determination. It was showed that the yellow rice wine contained amino acids with high nutritional value such as proline, leucine and lysine, etc. In addition, the total content of amino acids in yellow rice wine with different ages was different, and the trend of individual amino acid was different. Conclusion The method is suitable for quantitative and qualitative analysis of free amino acids in yellow rice wine in sufficient number of samples with a sample and fast pretreatment procedure, the determination result is accurate and reliable, that could meet requirements of daily inspection and supervision work