慢性HBV感染者外周血淋巴细胞亚群的特征性研究

目的 分析慢性HBV感染者外周血T细胞亚群的特征及病程不同阶段的变化及意义.方法 采用流式细胞分析技术对75例慢性HBV感染者的外周血T细胞亚群进行检测,分析其差异及意义并与正常对照组相比较.结果 乙肝肝硬化患者的外周血白细胞及淋巴细胞绝对数低于慢性乙肝患者、携带者及正常对照组:与正常对照组比较,慢性乙肝、肝硬化患者的外周血CD4 T细胞、CD8 T细胞、NK细胞计数减少,并且随病情的进展逐渐降低.结论 HBV感染者体内存在T淋巴细胞亚群失衡和细胞免疫功能紊乱.外周血T淋巴细胞亚群随病情进展而减少,主要为CD4 T细胞、CD8 T细胞、NK细胞的减少.

Characteristics of Peripheral Blood T -lymphocyte Subsets in Patients with Chronic Hepatitis B

Objective To investigate the characteristics of peripheral blood T-lymphocyte subsets in the patients with HBV infected and explore their relations with the disease state.Methods 75 patients with chronic HBV infected and twenty healthy individuals as controls were enrolled in our study.Flow cytometry was used to detect the peripheral blood T-lymphocytes.Results Compared with normal controls,the carriers and the patients with chronic hepatitis B,the numbers of lymphocytes decreased in cirrhosis.The number of CD4+ T cells,CD8+ T cells decreased in the patients with chronic hepatitis B and the cirrhosis compared with the normal and the carriers.Conclusion There is an imbalance in peripheral blood T-lymphocyte subsets and turbulence in cellular immunity in the patients with chronic hepatitis B,the absolute number of CD4+ T cells,CD8+ T cells and NK cells in the patients with chronic HBV infection decrease significantly and are correlated with the disease progression.