云南丽江产白雪茶多糖的分离提取与含量测定

采用水提醇沉法从云南丽江产白雪茶中分离提取出雪茶多糖（Thamnolia vermicularis polysaccharide,TP）,经石油醚、乙醇回流去除脂类、小分子杂质、Sevag法脱蛋白、透析后,用硫酸-苯酚法测定了多糖含量。结果表明,在10μg/ml-80μg/ml范围内,吸光度与被测物含量之间具有较好的线性关系（R2=0.9982）。重复性试验、回收率试验表明,该方法具有较好的重复性（RSD=2.47%）与准确度（RSD=2.76%）。测定结果表明,雪茶多糖中总糖含量为81.26%（RSD=2.13%）,雪茶原料中多糖含量为2.78%。

Isolation And Determination of Polysaccharide From Thamnolia Vermicularis Grown in Li Jiang,Yunnan

Using water extracting-alcohol precipitating method,Thamnolia vermicularis polysaccharide（TP）was isolated from Thamnolia vermicularis grown in LIjiang City,Yunnan province.The lipids and other small molecules impurities in Thamnolia vermicularis were removed by petroleum ether and ethanol reflux.The protein in TP was removed by Sevag＇s method.Then the total polysaccharide content of TP was determined by the method of phenol-sulfuric acid.The results showed that there is good linearity relation between the absorbency and the content in the range of 10μg/ml-80μg/ml（R2 = 0.9982）.The RSDs of repeatability and recovery tests were 2.47% and 2.76% respectively,which demonstrated the method had well repeatability and accuracy.The determination results showed that the total content of polysaccharide in TP was 81.26%（RSD=2.13%）and the content of polysaccharide in Thamnolia vermicularis was 2.78%.