Watershed-scale landuse is associated with temporal and spatial compositional variation in Lake Michigan tributary bacterial communities |
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| Authors: | Gabrielle E Sanfilippo Jared J Homola Jared Ross Jeannette Kanefsky Jacob Kimmel Terence L Marsh Kim T Scribner |
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| Affiliation: | 1. Department of Fisheries and Wildlife, 480 Wilson Road, 13 Natural Resources Building, Michigan State University, East Lansing, MI 48864, United States;2. Department of Microbiology and Molecular Genetics, 567 Wilson Road, 2215 Biomedical Physical Sciences Building, Michigan State University, East Lansing, MI 48864, United States;3. Department of Integrative Biology, 288 Farm Ln, Natural Sciences Building, Michigan State University, East Lansing, MI 48864, United States;1. Centre for Earth Observation Science, University of Manitoba, Winnipeg, MB, Canada;2. Department of Earth, Ocean and Atmospheric Sciences, University of British Columbia, Vancouver, BC, Canada;3. Department of Botany, University of British Columbia, Vancouver, BC, Canada;1. Microbiology Department, University of Manitoba, R3T 2N2 Winnipeg, MB, Canada;2. Chemistry Department, University of Manitoba, R3T 2N2 Winnipeg, MB, Canada;1. U.S. Geological Survey, Great Lakes Science Center, Tunison Laboratory of Aquatic Science, 3075 Gracie Road, Cortland, NY 13045, USA;2. U.S. Geological Survey, New York Cooperative Fish and Wildlife Research Unit, Cornell University, Ithaca, NY 14853, USA;3. SUNY Research Foundation, 3075 Gracie Road, Cortland, NY 13045, USA;1. Department of Fisheries and Wildlife, Michigan State University, 480 Wilson Rd, East Lansing, MI 48824, USA;2. Department of Integrative Biology, Michigan State University, 252 Farm Ln., East Lansing, MI 48824, USA;3. Ecology, Evolution, and Behavior Program, 103 Giltner Hall, 293 Farm Lane, East Lansing, MI 48824, USA;1. School of Geographic Sciences, Nantong University, Nantong, 226007, China;2. CAS Key Laboratory of Urban Pollutant Conversion, Department of Environmental Science and Engineering, University of Science & Technology of China, Hefei, 230026, China;3. Anhui Province Key Laboratory of Polar Environment and Global Change, University of Science & Technology of China, Hefei, 230026, China |
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| Abstract: | Populations of stream organisms across trophic levels, including microbial taxa, are adapted to physical and biotic stream features, and are sentinels of geological and hydrological landscape processes and anthropogenic disturbance. Stream bacterial diversity and composition can have profound effects on resident and migratory species in Great Lakes tributaries. Study objectives were to characterize and compare the taxonomic composition and diversity of bacterial communities in 18 rivers of the Lake Michigan basin during April and June 2019 and to quantify associations with stream and watershed physical features and dominant landuse practices. River water was filtered, and genomic DNA was extracted from filtrate using antiseptic techniques. We performed high-throughput amplicon sequencing using the highly variable V4 region of the 16S rRNA gene to characterize microbial community composition and diversity. Effects of landscape-scale landuse, environmental variables and dispersal predictors (e.g., inter-stream distance) on community compositional differences were quantified. Greater than 90% of variation in bacterial relative abundance between rivers and time were attributed to 11 phyla representing 10,800 operational taxonomic units. Inter-stream geographic distance, stream hydrology, and variation in stream properties that were tied to patterns of watershed landuse were significantly associated with differences in bacterial community composition among streams at both sampling time periods. based on Bray-Curtis distances. Understanding how environmental characteristics and watershed-scale landuse influence lower trophic level stream communities such as bacteria will inform managers as biological indicators of ecosystem health, sources of disturbance, and current and future bottom-up trophic changes in coupled tributary-Great Lakes ecosystems. |
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| Keywords: | Bacterial diversity Great Lakes tributaries Landuse Landscape ecology Watershed ecology Riverscapes |
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