| 碱性成纤维细胞生长因子受体mRNA基因在人晶体上皮细胞内的检测研究 |
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| 引用本文： | 刘玉福,袁佳琴,王德文,孙慧敏,施玉英,宋良文,李筱荣.碱性成纤维细胞生长因子受体mRNA基因在人晶体上皮细胞内的检测研究[J].中国实用眼科杂志,1999(5). |
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| 作者姓名： | 刘玉福 袁佳琴 王德文 孙慧敏 施玉英 宋良文 李筱荣 |
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| 作者单位： | 天津医科大学世界人工晶体中心中国天津培训中心，北京同仁医院眼科，军事医学科学院放射病理研究所 |
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| 摘 要： | 为研究碱性成纤维细胞生长因子（ｂａｓｉｃｆｉｂｒｏｂｌａｓｔｇｒｏｗｔｈｆａｃｔｏｒ，ｂＦＧＦ）对晶体上皮细胞促增殖作用的机理，采用原位杂交，用合成的寡核苷酸探针来检测人晶体上皮细胞内的ｂＦＧＦ高亲和力受体—成纤维细胞生长因子受体１（ｆｉｂｒｏｂｌａｓｔｇｒｏｗｔｈｆａｃｔｏｒｒｅ－ｃｅｐｔｏｒ１，ＦＧＦＲ１）的ｍＲＮＡ基因，并用图像分析进行相对定量。结果：表明了人晶体上皮细胞表达ＦＧＦＲ１的ｍＲＮＡ基因。结论：人晶体上皮细胞存在ＦＧＦＲ１，当ｂＦＧＦ与其高亲和力受体结合后，对促进晶体上皮细胞的增殖以及白内障术后后囊混浊的形成具有重要作用。
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| 关 键 词： | 人晶体上皮细胞 受体 基因 原位杂交 碱性成纤维细胞生长因子 |
The study on detection of mRNA gene of basic fibroblast growth factor receptor in
human lens epithelial cells |
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| Liu Yu fu,Yuan Jia qin,Wang De wen,et al..The study on detection of mRNA gene of basic fibroblast growth factor receptor in
human lens epithelial cells[J].Chinese Journal of Practical Ophthalmology,1999(5). |
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| Authors: | Liu Yu fu Yuan Jia qin Wang De wen |
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| Affiliation: | Liu Yu fu,Yuan Jia qin,Wang De wen,et al.
International Intraocular Implant Training Center,Tianjin Medical Uuiversity,Tianjin 300007 |
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| Abstract: | Objective:To study the mechanism of the promotive effects of basic fibroblast
growth factor (bFGF) on proliferation of lens epithelial cells.Methods:By in situ
hybridization,it was found a high affinity recptor of bFGF fibroblast growth factor
receptor1(FGFR1)in human lens epithelial cell using a synthesized oligonucleotide probe
for bFGF receptor and by using microimage analysis technique,the amounts of their
expression were analyed with relative quantitation.Results:We demonstrated that there
was mRNA gene expression of FGFR1.Conclusion:FGFR1 exists in lens epithelial
cells,and when bFGF is bound to its high affinity receptor,the binding plays and
important role in lens epithelial cell proliferation and capsular opacity after cataract
surgery. |
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| Keywords: | Human lens epithelial cells Receptor bFGF In situ hybridization |
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