| 面向工业化的裂殖壶菌DHA油脂提取方法研究 |
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| 引用本文： | 朱婧瑶,张红漫,胡耀池,胡学超,任路静.面向工业化的裂殖壶菌DHA油脂提取方法研究[J].食品科技,2011(9):32-35,40. |
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| 作者姓名： | 朱婧瑶 张红漫 胡耀池 胡学超 任路静 |
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| 作者单位： | 南京工业大学生物与制药工程学院材料化学工程国家重点实验室;南京工业大学理学院; |
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| 基金项目： | 国家自然科学基金项目(20936002); 国家重点基础研究发展计划(973计划)(2007CB707805;2009CB724700); 江苏省六大人才高峰项目(2008); 江苏省高校科研成果产业化推进项目(2009) |
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| 摘 要： | 研究液氮法、均质法、微波法、酶法等9种细胞破碎方法对裂殖壶菌单细胞油脂提取的影响,分别考察了各种细胞破碎方法提取得到的单细胞油脂含量、DHA含量、油脂中的脂肪酸分布以及耗时、耗能等情况,结果表明酶法和酸热法效果最好,其中酶法由于投入低、能耗少、操作简单等优点更具工业化潜力。将酶法与传统的均质法进一步放大对比,验证了酶法的优良性能。
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| 关 键 词： | 二十二碳六烯酸(DHA) 裂殖壶菌 细胞破碎 油脂提取 工业化 |
Comparation on lipid extraction methods of Schizochytrium sp. for industrialization |
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| ZHU Jing-yao,ZHANG Hong-man,HU Yao-chi,HU Xue-chao,REN Lu-jing.Comparation on lipid extraction methods of Schizochytrium sp. for industrialization[J].Food Science and Technology,2011(9):32-35,40. |
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| Authors: | ZHU Jing-yao ZHANG Hong-man HU Yao-chi HU Xue-chao REN Lu-jing |
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| Affiliation: | ZHU Jing-yao1,ZHANG Hong-man2*,HU Yao-chi1,HU Xue-chao1,REN Lu-jing1(1.Nanjing University of Technology,College of Pharmacy and Life Science,State Key Laboratory of Materials-Oriented Chemical Engineering,Nanjing 210009,2. Department of Applied Chemistry,Nanjing University of Technology,Nanjing 210009) |
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| Abstract: | Effect of different cell disruption methods, such as liquid nitrogen, homogenization, microwaves and enzymatic extraction on the extraction of single cell lipid of Schizochytrium sp. was determined. The lipid and DHA contents, fatty acid composition of the lipid, the time and energy consumption was studied when using different methods. The enzymatic and acid-heating extraction was the two most effective methods. Because of the low inputs, low energy consumption and simple operation, enzymatic extraction sho... |
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| Keywords: | Docosahexaenoic acid(DHA) Schizochytrium sp cell disruption lipid extration industrialization |
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