| 低氧和高氧对大菱鲆幼鱼红细胞核异常及氧化抗氧化平衡的影响 |
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| 引用本文： | 吴志昊,尤锋,王英芳,文爱韵,马得友,徐永立,张培军.低氧和高氧对大菱鲆幼鱼红细胞核异常及氧化抗氧化平衡的影响[J].上海水产大学学报,2011(6). |
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| 作者姓名： | 吴志昊 尤锋 王英芳 文爱韵 马得友 徐永立 张培军 |
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| 作者单位： | 中国科学院海洋研究所;中国科学院研究生院;青岛科技大学化工学院; |
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| 基金项目： | 国家科技支撑计划项目(2011BAD13B04) |
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| 摘 要： | 研究了极低氧(0.2、1.0、2.0 mg/L)、低氧(3.0、5.0 mg/L)和高氧(11.0、14.0 mg/L)处理28 d期间,大菱鲆幼鱼外周血红细胞核异常以及血清超氧化物岐化酶(SOD)、过氧化氢酶(CAT)活力和肝脏丙二醛(MDA)含量等氧化抗氧化平衡中参数的变化。结果显示,极低氧条件下,0.2、1.0和2.0 mg/L组的实验鱼分别在处理2 h、1 d和2 d内全部死亡,其余各组则没有出现死亡现象。溶解氧(DO)对大菱鲆核异常包括微核及总核异常率的影响表现出明显的时间和剂量累积效应,到28 d时才在5.0、14.0 mg/L组和对照组中(正常充气,约7.0 mg/L)观察到微核现象。恢复正常充氧14 d后,高氧组和对照组中仍有微核现象出现。2 h时极低氧组SOD活力较高。其他各处理时间时,SOD活力在各组之间差异显著。各组CAT活力在2 h时未发生明显变化,在7、28和42 d时差异显著(P<0.05)。极低氧组大菱鲆肝脏MDA含量较低,低氧组和高氧组MDA含量均随着处理时间的延长逐渐降低。恢复后低氧组MDA含量显著升高(P<0.05)。研究还发现,对照组大菱鲆也会受到过氧化的胁迫作用,而5....
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| 关 键 词： | 大菱鲆幼鱼 溶解氧 核异常 超氧化物歧化酶 过氧化氢酶 丙二醛 |
The effects of hypoxia and hyperoxia on nucleus anomaly,SOD,CAT activities and MDA content in juvenile turbot Scophthalmus maximus |
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| WU Zhi-hao,YOU Feng,WANG Ying-fang,WEN Ai-yun,MA De-you,XU Yong-li,ZHANG Pei-jun.The effects of hypoxia and hyperoxia on nucleus anomaly,SOD,CAT activities and MDA content in juvenile turbot Scophthalmus maximus[J].Journal of Shanghai Fisheries University,2011(6). |
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| Authors: | WU Zhi-hao YOU Feng WANG Ying-fang WEN Ai-yun MA De-you XU Yong-li ZHANG Pei-jun |
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| Affiliation: | WU Zhi-hao1,2,YOU Feng1,WANG Ying-fang3,WEN Ai-yun1,MA De-you1,XU Yong-li1,ZHANG Pei-jun1(1.Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,Shandong,China,2.Graduate University,Beijing 100039,3.College of Chemical Engineering,Qingdao University of Science and Technology,Qingdao 266042,China) |
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| Abstract: | The effects of different dissolved oxygen(DO) concentrations,including severe hypoxia(0.2,1.0,2.0 mg/L),hypoxia(3.0,5.0 mg/L),normoxia(normal aeration as control,about 7.0 mg/L) and hyperoxia(11.0,14.0 mg/L),on nucleus anomaly in erythrocytes,superoxide dismutase(SOD),catalase(CAT) activities in serum and malondialdehyde(MDA) concentration in liver were studied in juvenile turbot Scophthalmus maximus.Fish mortality was only observed in the 0.2,1.0 and 2.0 mg/L groups,in which all fish died in 2 h,24 h and 4... |
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| Keywords: | juvenile turbot Scophthalmus maximus dissolved oxygen nucleus anomaly superxide dismutase activities catalase activities malondialdehyde content |
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