| 溶组织梭状芽孢杆菌胶原蛋白酶的研究进展 |
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| 引用本文： | 肖寒,刘秀妨,郑淋,赵谋明,黄明涛. 溶组织梭状芽孢杆菌胶原蛋白酶的研究进展[J]. 食品科学, 2022, 43(17): 316-325. DOI: 10.7506/spkx1002-6630-20210830-385 |
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| 作者姓名： | 肖寒 刘秀妨 郑淋 赵谋明 黄明涛 |
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| 作者单位： | （华南理工大学食品科学与工程学院,广东 广州 510641） |
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| 基金项目： | 国家自然科学基金青年科学基金项目（31901013）;广东省重点领域研发计划项目（2020B020226009）;广州市科技计划项目（202002030337） |
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| 摘 要： | 胶原蛋白是哺乳动物体内含量最丰富的蛋白质之一,因具有特殊的三股螺旋结构而不易被普通蛋白酶水解。胶原蛋白酶可在生理条件下特异性识别切割胶原蛋白,在医药、食品等工业领域应用广泛,其中以源自溶组织梭状芽孢杆菌的胶原蛋白酶研究较多。本文主要对来自溶组织梭状芽孢杆菌的胶原蛋白酶（collagenase,Col）G和ColH的结构特征、水解机理、生产现状和实际应用等方面进行概括总结,以期为胶原蛋白酶的进一步发展和利用提供理论参考依据。
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| 关 键 词： | 溶组织梭状芽孢杆菌 胶原蛋白酶 水解机理 异源表达, |
Recent Advances in Collagenase from Clostridium histolyticum |
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| XIAO Han,LIU Xiufang,ZHENG Lin,ZHAO Mouming,HUANG Mingtao. Recent Advances in Collagenase from Clostridium histolyticum[J]. Food Science, 2022, 43(17): 316-325. DOI: 10.7506/spkx1002-6630-20210830-385 |
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| Authors: | XIAO Han LIU Xiufang ZHENG Lin ZHAO Mouming HUANG Mingtao |
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| Affiliation: | (School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China) |
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| Abstract: | Collagen, one of the most abundant proteins in mammals, is hardly degraded by conventional proteases due to its special triple-helix structure. Collagenase is able to cleave collagen under physiological conditions, which has been widely applied in the medicinal and food industries. A lot of research has been done on collagenase from Clostridium histolyticum. In this review, we summarize the structural characteristics, the collagenolytic mechanism, the current status of production, and the application of collagenase (Col) G and ColH from Clostridium histolyticum in order to provide a rationale for further development and utilization of collagenase. |
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| Keywords: | Clostridium histolyticum collagenase collagenolytic mechanism heterologous expression, |
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