蛋白沉淀-HPLC法测定大鼠血浆中多西他赛的含量

目的建立大鼠血浆中多西他赛含量测定的方法。方法采用蛋白沉淀-HPLC法。色谱柱:Hypersil C18柱(250 mm×4.6 mm,5μm);沉淀剂:体积分数80%的甲醇;流动相:甲醇-乙腈-水(体积比35∶40∶25);流速:1 mL.min-1;检测波长:233 nm。结果血浆中多西他赛检测方法的线性范围为0.05~50.0 mg.L-1,定量下限为50μg.L-1;血浆中多西他赛的提取回收率为87.80%,日内RSD<5.5%,日间RSD<8.5%。结论本法可用于多西他赛的药物动力学研究。

Content determination of docetaxel in rat plasma by protein precipitation and HPLC method

Abstract：Objective To develop a protein precipitation and HPLC method for the determination of docetaxel concentration in rat plasma. Methods Protein was precipitated by 80 % methanol , and a Hypersil C18 column (250 mm×4.6 mm,5 µm) used with a mobile phase consisting of methanol-acetonitrile-water (V:V:V=35:40:25) at the detection wavelength of 233 nm. Results The linear range of the plasma concentration of docetaxel was 0.05-50.0 mg·L-1 and the limit of detection was 50 µg·L-1．The recoveries of docetaxel were 87.80 %. The RSD of intra-day and inter-day were less than 5.5 % and 8.5 % ,respectively．Conclusion The method has high sensitivity and good selectivity; it is suitable for the pharmacokinetic research of docetaxel in rat plasma.