蓝莓复合果汁饮料对兔干眼症模型影响的实验研究

目的：观察蓝莓复合果汁饮料对实验性兔干眼症模型的缓解效果。方法：新西兰兔30只（60只眼），随机分为空白对照组，模型对照组，蓝莓低、中、高剂量组和阳性对照组，每组5只（10只眼）。除空白对照组外，其余组动物双眼滴0．25％氯霉素滴眼液（每次每眼3滴，每天2次）并适应性喂养3d后切除泪腺制作干眼症模型。手术后第8天起，各组予相应药物进行治疗，连续4周。治疗的同时各组每周进行1次SchirmerI试验、泪膜破裂时间记录及角膜结膜虎红染色情况观察，比较各组结果。结果：各给药组动物SchirmerI试验滤纸湿长度和泪膜破裂时间分别在用药3周及4周后与模型对照组比较显著增加（P〈0．05，P〈0．01）；蓝莓高剂量组在用药2周后、其他给药组在用药3周后虎红染色点数与模型组比较显著减少（P〈0．05，P〈0．01）。结论：蓝莓复合果汁饮料对实验性干眼症有改善作用。

Experimental study on therapeutic effect of compound blueberry juice drink in rabbit model of xerophthalmia

Objective： To observe the therapeutic effect of compound blueberry juice drink in a rabbit model of xerophthalmia. Methods：Thirty New Zealand rabbits （60 eyes） were randomly and equally divided into blank control group, model control group,low-, middle-, and high- dose treatment groups （to be given compound blueberry juice drink ）,and positive control group. In all rats except those in blank control group,0. 25% chloramphenicol eye drops （3 drops/eye） were given to both eyes twice daily,and adaptive feeding was performed for 3 d;then,the lacrimal glands were excised to establish a rabbit model of xerophthalmia. Since the 8th day after operation,the treatment groups received respective treatments for 4 weeks. During treatment,Schirmer I test,recording of tear break - up time （ TBUT）, and rose bengal staining of the cornea and conjunctiva were performed in each group once a week, and the results were compared. Results：Compared with the model control group, all treatment groups had a significantly increased moistening length of filter paper in Schirmer I test and a significantly prolonged TBUT after 3 and 4 weeks of treatment （P 〈0. 05 or P 〈0. 01 ） ; the high - dose treatment group had a significantly reduced number of dots stained by rose bengal after 2 weeks of treatment, and other treatment groups had a significantly reduced number of dots stained by rose bengal after 3 weeks of treatment （P 〈 0. 05 or P 〈 0. 01 ）. Conclusion：Compound blueberry juice drink has therapeutic effect in rabbits with experimental xerophthalmia.