变形链球菌表面蛋白和葡糖基转移酶基因疫苗对唾液变形链球菌和牙菌斑的影响

目的　了解变形链球菌表面蛋白和葡糖基转移酶基因疫苗单独及联合免疫对定菌鼠唾液变链菌和牙面菌斑的影响。方法　 2 8d龄 Wistar大鼠 3 6只 ,随机分为 pc DNA3 - pac组、pc DNA3 - gtf B组、pc DNA3 - pac联合pc DNA 3 - gtf B组、变形链球菌灭活全菌组、pc DNA3空载体组和 PBS液组 ,进行三次双侧颌下腺腺周注射免疫 ,建立定菌鼠模型 ,作诱龋实验 3个月。唾液变链菌计数和菌斑计分。结果　唾液变链菌菌落计数和牙面菌斑计分在 pc D-NA3与 PBS组最高 ,其次为单基因疫苗免疫组 ,联合基因疫苗和灭活全菌细胞免疫组最低 ,各组间有显著性差异 ( P<0 .0 5 )。结论　 pc DNA3 - gtf B和 pc DNA3 - pac具有明显的免疫抑菌作用 ,联合基因疫苗免疫优于单基因疫苗

The Influence of Gene Vaccines pcDNA3-pac and pcDNA3-gtfB of Streptococcus mutans on Plaque Index and S. mutans Score in Rats

OBJECTIVE: This study was aimed at using the gene vaccines pcDNA3-pac and pcDNA3-gtfB which were constructed on PAc and GTF-B gene vaccinating gnotobiotic rats by submandibular gland-target injection to observe the level of salivary S. mutans and dental plaque inhabitation. METHODS: Thirty-six 28-day-old Wistar rats were divided into 6 groups, including those vaccinated with pcDNA3-pac, pcDNA3-gtfB, pcDNA3-pac combined with pcDNA3-gtfB, inactive whole cell of S. mutans JBP, and those injected with pcDNA3 and 0.1 mol/L PBS buffer respectively. Each rat was administrated with 100 micrograms vaccines or controlled materials by submandibular gland-target injection, vaccinated three times at 1-2 weeks interval. Then the gnotobiotic rat model was constructed, following that all rats were fed with cariogenic diet 2000# for 3 months. Rat saliva was collected to assay the level of S. mutans in saliva and evaluate the dental plaque index. RESULTS: The level of S. mutans in saliva and the dental plaque index of the group vaccinated with pcDNA3-pac combined with pcDNA3-gtfB and the group with inactive S. mutans cell were lower than those of other groups. And they were the highest in the groups injected with pcDNA3 or PBS buffer (P < 0.01 or P < 0.05). No statistically significant differences were observed between the group vaccinated with pcDNA3-pac and the group vaccinated with pcDNA3-gtfB, between the group vaccinated with pcDNA3-pac combined with pcDNA3-gtfB and the group for positive control, and between the pcDNA3 group and PBS buffer group (P > 0.05). CONCLUSION: The two gene anticaries vaccines induce the specific antibodies and thus produce effects on the level of S. mutans in saliva and dental plaque index. Therefore gene vaccines pcDNA3-pac and pcDNA3-gtfB are effective, and joint gene vaccines inoculation is optimal.