Susceptibility of Epithelial Cells to Pseudomonas aeruginosa Invasion and Cytotoxicity Is Upregulated by Hepatocyte Growth Factor |
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| Authors: | Suzanne M. J. Fleiszig Vicky Vallas Cindy H. Jun Leo Mok Daniel F. Balkovetz Michael G. Roth Keith E. Mostov |
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| Affiliation: | The Morton D. Sarver Laboratory for Cornea and Contact Lens Research, School of Optometry, University of California, Berkeley,1. and Department of Anatomy and Biochemistry, University of California, San Francisco,2. California, and University of Texas Southwestern Medical Center at Dallas, Dallas, Texas3. |
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| Abstract: | Normal cell polarity protects epithelial cells against Pseudomonas aeruginosa invasion and cytotoxicity. Using epithelial cell clones with selective defects in sorting of membrane constituents, and using hepatocyte growth factor pretreatment, we found that polarized susceptibility to P. aeruginosa can be altered without disrupting tight junctions. The results also showed that cellular susceptibility factors for invasion and cytotoxicity are not the same, although both are localized to the basolateral cell surface in polarized epithelial cells. |
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