化学合成 microRNA-21 inhibitors 对大鼠心肌成纤维细胞活化增殖的影响

目的：探究 microRNA-21 inhibitors 对大鼠心肌成纤维细胞活化增殖的影响。方法应用 LipofectamineTM 2000 Reagent 向大鼠心肌成纤维细胞瞬时转染 microRNA-21 in-hibitors 24、48 h 后,实时荧光定量聚合酶链反应(qRT-PCR)检测 microRNA-21、I 型胶原前胶原 A1(Col1A1)和α-平滑肌肌动蛋白(α-SMA)的表达水平；Western blot 法检测 Col1A1和α-SMA 的表达水平；MTT 法检测 microRNA-21 inhibitors对心肌成纤维细胞活化增殖的影响。结果转染 microR-NA-21 inhibitors 的心肌成纤维细胞中,microRNA-21表达下调,Col1A1和α-SMA 的表达下降；转染 microRNA-21 inhibi-tors 的心肌成纤维细胞增殖活性明显减弱。结论 microR-NA-21 inhibitors 可明显抑制心肌成纤维细胞增殖活性,提示microRNA-21是心肌成纤维细胞活化增殖的潜在靶向分子,有利于为干预和预防心肌纤维化的发生发展提供新思路。

The effect of microRNA-21 inhibitors on proliferation and activation of cardiac fibroblasts in rats

Objective To investigate the effect of microRNA-21 inhibitors on proliferation and activation of cardiac fibroblasts in rats. Methods LipofectamineTM 2000 Reagent was used to transfected cardiac fibroblasts with mi-croRNA-21 inhibitors. After 24,48 h, qRT-PCR was applied to assess the expressions of microRNA-21 and mRNA of Col1A1 and α-SMA. The protein expression levels of Col1A1 and α-SMA were detected by Western blot. MTT assay was used to determine the proliferation influence of the transfected cardiac fibroblasts. Results The cardiac fibroblasts transfected microRNA-21 inhibitors exhibited down-regulated microRNA-21 expression and attenuated Col1A1 and α-SMA mRNA expression. The cardiac fibroblasts proliferation activity decreased significantly after transfected microRNA-21 inhibitors. Conclusion MicroRNA-21 inhibitors can suppress the proliferation activity of cardiac fibroblasts significantly, implicating microRNA-21 as a potential target for cardiac fibroblasts activation and proliferation and pointing out that this result can provide a new idea for intervening and preventing the myocardial fibrosis occurrence and development.