| 采用全烟气暴露系统测试卷烟主流烟气细胞毒性 |
| |
| 引用本文： | 李翔,聂聪,尚平平,谢复炜,刘惠民,谢剑平. 采用全烟气暴露系统测试卷烟主流烟气细胞毒性[J]. 烟草科技, 2012, 0(5): 44-47 |
| |
| 作者姓名： | 李翔 聂聪 尚平平 谢复炜 刘惠民 谢剑平 |
| |
| 作者单位： | 中国烟草总公司郑州烟草研究院,郑州高新技术产业开发区枫杨街2号,450001 |
| |
| 基金项目： | 国家自然科学基金资助项目，国家烟草专卖局科技项目，国家烟草专卖局标准项目 |
| |
| 摘 要： | 基于VITROCELL系统结合中性红摄取试验建立了全烟气暴露细胞毒性测试方法,采用建立的方法测试了加拿大深度(HCI)抽吸条件下国内外29个牌号市售卷烟主流烟气的细胞毒性。结果表明:①建立的全烟气暴露细胞毒性测试方法可以获得良好的烟气细胞毒性剂量-效应关系;②测试结果的变异系数(CV)在25%以内,方法稳定性较好。建立的全烟气暴露实验方法适合于卷烟主流烟气细胞毒性测试。
|
| 关 键 词： | 卷烟烟气 全烟气 HCI抽吸条件 中性红 细胞毒性 |
In vitro Cytotoxicity Assay for Mainstream Cigarette Smoke with Whole Smoke Exposure System |
| |
| LI Xiang , NIE Cong , SHANG Pingping , XIE Fuwei , LIU Huimin , XIE Jianping. In vitro Cytotoxicity Assay for Mainstream Cigarette Smoke with Whole Smoke Exposure System[J]. Tobacco Science & Technology, 2012, 0(5): 44-47 |
| |
| Authors: | LI Xiang NIE Cong SHANG Pingping XIE Fuwei LIU Huimin XIE Jianping |
| |
| Affiliation: | Zhengzhou Tobacco Research Institute of CNTC,Zhengzhou 450001,China |
| |
| Abstract: | The method of in vitro cytotoxicity assay for mainstream cigarette smoke was established by using VITROCELL? whole smoke exposure system associated with neutral red uptake assay.The cytotoxicity of mainstream cigarette smoke of 29 domestic and imported commercial available brands was evaluated by this method under Health Canada Intensive(HCI)smoking regime.The results indicated that: 1)A well dose-response relationship between cigarette smoke doses and cell viability could be obtained by the established method.2)The stability of the method was satisfactory with the values of CV below 25%.The established method of whole smoke exposure could be applied to evaluate the cytotoxicity of mainstream cigarette smoke. |
| |
| Keywords: | Cigarette smoke Whole smoke HCI smoking regime Neutral red Cytotoxicity |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
