| 海嘧啶对小鼠S180和EAC肿瘤细胞 P53蛋白表达的影响 |
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| 引用本文： | 王志举,乔鹏,章茜,季宇彬.海嘧啶对小鼠S180和EAC肿瘤细胞 P53蛋白表达的影响[J].郑州大学学报(医学版),2003,38(5):751-752. |
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| 作者姓名： | 王志举 乔鹏 章茜 季宇彬 |
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| 作者单位： | 1. 郑州大学基础医学院生理学教研室,郑州,450052
2. 哈尔滨商业大学制药工程系,哈尔滨,150076 |
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| 基金项目： | 国家科委生命中心博士基金资助课题 96-901-06-10 |
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| 摘 要： | 目的观察海嘧啶对小白鼠S180肿瘤和EAC肿瘤细胞P53蛋白表达的影响.方法将含有传代后的小鼠S180肿瘤细胞和EAC肿瘤细胞的培养瓶各分为A,B1,B2,B3,B4 5组,B1～ B4组分别加入海嘧啶,使培养液中药物的终浓度分别为50 mg/L、100 mg/L、200 mg/L、400 mg/L,A组加入等体积的培养液(对照组).用Western blot法测定瘤细胞P53蛋白表达.结果S180肿瘤细胞和EAC肿瘤细胞P53蛋白表达率分别是100%和90%;海嘧啶100～400 mg/L时能抑制肿瘤细胞P53蛋白的表达,与对照组比较,差异有统计学意义(P<0.05).结论海嘧啶抗肿瘤的药理机制与其能够抑制肿瘤细胞突变型P53蛋白表达有关.
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| 关 键 词： | 海嘧啶 肿瘤细胞 P53蛋白 小鼠 EAC肿瘤细胞 S180肿瘤细胞 |
| 修稿时间： | 2002年10月29 |
Influence of sea pyrimidine on expression of P53 protein of mouse S180 and EAC tumor cells |
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| WANG Zhiju ,QIAO Peng ,ZHANG Qian ,JI Yubin.Influence of sea pyrimidine on expression of P53 protein of mouse S180 and EAC tumor cells[J].Journal of Zhengzhou University: Med Sci,2003,38(5):751-752. |
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| Authors: | WANG Zhiju QIAO Peng ZHANG Qian JI Yubin |
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| Affiliation: | WANG Zhiju 1),QIAO Peng 1),ZHANG Qian 1),JI Yubin 2) 1)Department of Physiology,School of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450052 2)Department of Chinese Medicine,Ha' erbin Commercial University,Ha' erbin 150076 |
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| Abstract: | Aim: To investigate the influence of sea pyrimidine on the expression of P53 protein of mouse S180 and EAC tumor cells.Methods:S180 tumor cells and EAC tumor cells were allocated into 5 groups (groups A, B 1,B 2, B 3,and B 4), respectively and sea pyrimidine with different doses were added to groups B 1 to B 4, the expression of P53 protein was detected by Western blot.Results: The positive expression rate of P53 protein were 100% in S180 tumor cells and 90% in EAC tumor cells, and sea pyrimidine with effective concentration inhibited the expression of P53 protein of mouse tumor cells.Conclusion: The anti tumor pharmacologic mechanism of sea pyrimidine is related to its inhibiting the expression of P53 protein of mouse tumor cells. |
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| Keywords: | sea pyrimidine tumor cells P53 protein mouse |
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