干扰素诱导淋巴瘤细胞凋亡实验及临床研究

目的：分析梯度浓度的干扰素(IFN-α)对Burkitt淋巴瘤细胞株Daudi和T细胞淋巴瘤细胞株Jurkut及15例难治性淋巴瘤患者的直接作用。方法：以MTT法测定梯度浓度的IFN-α对两种淋巴瘤细胞株Daudi、Jurkat增殖作用的影响，以DNA末端标记法，流式细胞术，电镜观察测定IFN-α对淋巴瘤细胞的凋亡诱导作用，并采用瘤内注射IFN-α联合化疗治疗15例耐药的难治性淋巴瘤。结果：低浓度亚IFN-α对DaudiJurkat细胞增殖无明显抑制作用，高浓度IFN(10000U／ml)可显著抑制两种细胞增殖，且有时间相关性。高浓度的IFN-α可诱导淋巴瘤细胞凋亡。15例患者CR5例，PR7例，有效率80％，无明显毒副作用。结论：IFN-α可抑制淋巴瘤细胞增殖，诱导凋亡，有显著时间，剂量依赖性。局部应用IFN-α联合化疗是治疗难治性淋巴瘤的有效方法之一。

Experimental and clinical study on interferon-α inducing apoptosis of lymphoma cells

Objective:To study the direct effects of gradient concentration inteiferon-a(IFN-a) on lymphoma cells line Daudi and Jur-kat and a group of 15 cases refractory lymphoma. Methods: The effects of IFN-a on the growth of tumor cells were assayed by MTT, apoptosis were measured by flow cytometty, TUNEL and electron-microscope, 15 refractory lymphoma cases were treated by local injection of IFN-a combination chemotherapy.Results: Low-dose IFN-a did not have any significant effects on growth of Daudi and Jurkat cells, high-dose IFN-a (10 000U/ml) not only have significant anti-proliferative effects with dependence of time and dosage but also can induce apoptosis on both Daudi and Jurkat cells. 5 patients achieved a complete remission and 7 patients achieved a partial remission, the overall response rate was 80% .Conclusion:IFN-a( 10 000 U/ml) had significant anti-proliferative effects and inducing apoptosis on lymphoma cells.Local injection of IFN-a is one of effective therapy on refractory lymphoma.