小鼠2细胞期胚胎移植方法的探讨

目的以近交系C57BL/6J小鼠作为供体胚小鼠，封闭群ICR小鼠及昆明鼠作为假孕体，对2细胞期的胚胎移植方法和假孕鼠品系进行对比探讨。方法经超数排卵的C57BL/6J雌鼠于正常雄鼠交配，取受精卵，培养到2细胞期，进行输卵管伞移植和输卵管壁移植，妊娠产仔。结果从KM为假孕鼠，对2细胞期胚胎进行的输卵管壁移植的妊娠数量最多18只，90%的妊娠率，产仔数最多188只，产仔率达34.8%。结论输卵管壁移植的技术简单易行具有较高的妊娠率及产仔率。

Study on Methods of 2-Cell Embryo Transfer in Mice

Objective To compare and discuss the embryo transfer methods and the strains of foster mother by using 2- cell embryos from C 57 BL/6J mouse, ICR and Kunming mice as foster mother. Method Each of superovulated female C 57 BL/ 6J mice was mated individually with a stud male. The fertilized eggs were collected and cultured to 2-cell stage, then were transferred to pseudopregnant recipients. Results Using KM mice as foster mother, 2-cell embryos transferred through the wall of the fallopian tube, the pregnancy rate was 90% . The litter size was 188, rate of full-term young pups was 34.8%. Conclusion The approach of 2-cell embryos transferred through the wall of fallopian tube improve the efficiency of embryo transfer.