ATRA诱导神经母细胞瘤细胞分化及TrkB表达的研究 |
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引用本文: | 佟海侠,张锦华,张继红.ATRA诱导神经母细胞瘤细胞分化及TrkB表达的研究[J].中国小儿血液与肿瘤杂志,2001,6(4):150-152. |
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作者姓名: | 佟海侠 张锦华 张继红 |
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作者单位: | 1. 中国医科大学第二临床学院,检验科,110003 2. 中国医科大学第二临床学院,血液研究室,110003 |
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摘 要: | 通过对神经母细胞瘤 (neuroblastoma,NB)细胞系SMS KCNR的体外实验 ,探讨全反式维甲酸 (ATRA)对NB细胞增殖抑制的可能分子机制及BDNF(脑源性神经
营养因子 ) TrkB信号转导途径在NB细胞分化中的作用。通过台盼蓝拒染计数活细胞 ;用倒置相差显微镜观察加药处理前后细胞形态学变化 ;通过Northern印迹杂交来分析TrkB基因表达情况的改变。结果 :5μMATRA抑制NB细胞系SMS KCNR细胞增殖 ,而 5nMATRA无此作用 ;ATRA可诱导SMS KCNR细胞分化成熟 ;细胞分化过程中伴随TrkB基因表达水平增高。结果显示 :5μMATRA对人NB细胞系MSM KCNR细胞的体外增殖有抑制作用 ;ATRA能诱导人NB细胞系SMS KCNR细胞分化成熟 ;TrkB基因表达水平的增高可能是ATRA体外诱导NB细胞分化逆转的分子生物学机制之一。
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关 键 词: | 神经母细胞瘤 全反式维甲酸 分化 TrkB基因 Northern Blo |
Study on the induced differentiation of human NB cells and TrkB expression by ATRA |
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Abstract: | To investigate the possible molecular mechanisms of ATRA induced growth inhibition and the effect of BDNF TrkB signal transduction pathway on NB cell differentiation. Methods: Trypan blue exclusion is used to count the number of alive cells; Morphological differentiation is observed under the phase contrast microscope; Expression of TrkB gene before and after treatment of ATRA is analyzed using Northern blot analysis. Results: 5um ATRA but not 5nm ATRA inhibited NB cell growth; ATRA induced morphological differentiation of KCNR cells; The expression of TrkB gene was changed and the level of Trkb mRNA was increased during cell differentiation. Conclusion: 5um ATRA inhibited SMS KCNR NB cells growth; ATRA induced morphological differentiation of KCNR cells; The increase of TrkB gene expression is one of the molecular mechanisms of ATRA induced differentiation of human NB cells. |
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