| Mac-1基因缺失对黑色素瘤生长的影响 |
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| 引用本文： | 陈坚,段有发,叶志金,王丽京,章倩倩. Mac-1基因缺失对黑色素瘤生长的影响[J]. 中国比较医学杂志, 2017, 27(10): 23-27 |
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| 作者姓名： | 陈坚 段有发 叶志金 王丽京 章倩倩 |
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| 作者单位： | 广东药科大学基础学院, 血管生物学研究所, 广州 510006,广东药科大学基础学院, 血管生物学研究所, 广州 510006,广东药科大学基础学院, 血管生物学研究所, 广州 510006,广东药科大学基础学院, 血管生物学研究所, 广州 510006,广东药科大学基础学院, 血管生物学研究所, 广州 510006 |
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| 基金项目： | 国家自然科学基金（31712904）；广东省科技计划（2015A030302083）；广东省优秀青年教师培养计划（YQ2015100）；广州市珠江科技新星（201610010045）。 |
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| 摘 要： | 目的探讨Mac-1缺失对黑色素瘤肿瘤生长的影响。方法 Mac-1基因敲除(Mac-1-/-)小鼠扩群获得实验所需数量小鼠。设立对照组C57BL/6J小鼠和实验组Mac-1-/-小鼠,分别皮下注射B16-F10细胞,统计小鼠生存率、测量肿瘤体积和重量,通过免疫组化染色检测肿瘤细胞增殖及巨噬细胞浸润情况。结果 Mac-1缺失后的黑色素瘤小鼠存活率较对照组C57BL/6J小鼠有显著性差异(P﹤0.001),肿瘤体积和重量较对照组C57BL/6J小鼠有显著性差异(P﹤0.001),肿瘤组织中增殖细胞数目与对照组相比较有显著性差异(P﹤0.01),并且肿瘤组织中巨噬细胞浸润显著减少。结论 Mac-1基因缺失显著抑制黑色素瘤肿瘤生长。
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| 关 键 词： | Mac-1 黑色素瘤 肿瘤生长 |
| 收稿时间： | 2016-12-16 |
Effect of Mac-1 deficiency on the tumor growth of melanoma in mice |
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| CHEN Jian,DUAN You-f,YE Zhi-jin,WANG Li-jing and ZHANG Qian-qian. Effect of Mac-1 deficiency on the tumor growth of melanoma in mice[J]. Chinese Journal of Comparative Medicine, 2017, 27(10): 23-27 |
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| Authors: | CHEN Jian DUAN You-f YE Zhi-jin WANG Li-jing ZHANG Qian-qian |
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| Affiliation: | Institute of Vascular Biology, School of Basic Course, Guangdong Pharmaceutical University, Guangzhou 510006, China,Institute of Vascular Biology, School of Basic Course, Guangdong Pharmaceutical University, Guangzhou 510006, China,Institute of Vascular Biology, School of Basic Course, Guangdong Pharmaceutical University, Guangzhou 510006, China,Institute of Vascular Biology, School of Basic Course, Guangdong Pharmaceutical University, Guangzhou 510006, China and Institute of Vascular Biology, School of Basic Course, Guangdong Pharmaceutical University, Guangzhou 510006, China |
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| Abstract: | Objective To explore the influence of Mac-1 deficiency on tumor growth of melanoma. Methods The population of Mac-1 gene knock-out (Mac-1-/-) mice was expanded. B16-F10 cells were subcutaneously injected into the C57BL/6J mice (control group) and Mac-1-/-mice (experiment group), respectively. Subsequently,the survival rate, tumor volume and body weight were recorded. The proliferation and infiltration of macrophages were detected by immunohistochemistry. Results The survival rate of Mac-1-/- mice was significantly improved compared with the C57BL/6J mice (P<0.001). The tumor volume and body weight were remarkably decreased in the Mac-1-/- mice compared with the control group (P<0.001). Meanwhile, the tumor cell proliferation index was decreased in the Mac-1-/- mice compared with the control group (P<0.01).Furthermore, the infiltration of macrophages in the tumor tissues was also decreased in Mac-1-/- tumor mice compared with control group. Conclusions Mac-1 gene deletion can significantly suppress melanoma growth. |
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| Keywords: | Mac-1 Melanoma Tumor growth Mice |
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