附子配伍甘草后对附子生物碱类成分大鼠在体肠吸收特性的影响

目的:研究附子-甘草配伍前后附子中6种生物碱类成分的大鼠在体肠吸收特征。方法:采用大鼠在体肠灌注模型,以紫外分光光度法测定酚红质量浓度,利用LC-MS测定灌注液中乌头碱、次乌头碱、新乌头碱、苯甲酰乌头原碱、苯甲酰次乌头原碱、苯甲酰新乌头原碱的质量浓度,分别研究附子单煎液、附子-甘草合煎液、附子-甘草合并液3种配伍方式对上述6种生物碱类成分肠吸收的影响。结果:与附子单煎液相比,苯甲酰乌头原碱在附子-甘草合煎液、附子-甘草合并液中的吸收速率常数(Ka)和单位时间吸收率(A)呈下降趋势;苯甲酰新乌头原碱在附子-甘草合煎液、附子单煎液中的Ka和A相比附子-甘草合并液有所减少;附子-甘草合煎液中次乌头碱的Ka和A相比附子单煎液呈下降趋势,附子-甘草合并液中次乌头碱的Ka和A相比附子单煎液均呈升高趋势,附子-甘草合煎液中苯甲酰次乌头原碱、乌头碱和新乌头碱的吸收相比附子单煎液中均有所降低。结论:附子配伍甘草后6种乌头碱类成分在大鼠十二指肠中的吸收均有一定程度的降低,为阐释"附子得甘草而后缓"的配伍理论提供了实验依据。

Effect of Aconiti Lateralis Radix Praepareta Combined with Glycyrrhizae Radix et Rhizoma on in situ Intestinal Absorption of Alkaloids in Aconiti Lateralis Radix Praepareta

Objective:To study on the intestinal absorption of six alkaloids in Aconiti Lateralis Radix Praepareta(ALRP)-Glycyrrhizae Radix et Rhizoma(GRR) before and after their compatibility by in situ perfusion method in rats. Method:A rat intestinal perfusion model was used,the mass concentration of phenol red was measured by ultraviolet spectrophotometry,and the contents of aconitine,hypaconitine,mesaconitine,benzoylaconine,benzoylhypaconine,benzoylmesaconine in the perfusate were measured by LC-MS.Effect of three modes of compatibility on intestinal absorption of six alkaloids in ALRP decoction,ALRP-GRR combined decoction and ALRP-GRR separated decoction were investigated. Result:Compared with ALRP decoction,absorption rate constant(K_a) and absorption rate per unit time(A) of benzoylaconine in ALRP-GRR combined decoction and ALRP-GRR separated decoction showed a downward trend.Compared with ALRP-GRR separated decoction,K_a and A of benzoylmesaconine in ALRP-GRR combined decoction and ALRP decoction was decreased.Compared with ALRP decoction,K_a and A of hypaconitine in ALRP-GRR combined decoction showed a decreasing trend,K_a and A of hypaconitine in ALRP-GRR separated decoction showed a increasing trend.Compared with ALRP decoction,absorption of benzoylhypacoitine,aconitine and mesaconitine in ALRP-GRR combined decoction was in decline. Conclusion:Absorption of six alkaloids from ALRP in duodenum of rats decreases to some extent after combined with GRR.This study provides an experimental basis for the interpretation of the compatibility theory of "ALRP used with GRR can be absorbed slowly".