APPL1与新诊断2型糖尿病的相关性

摘要：目的测定新诊断2型糖尿病患者血清磷酸酪氨酸衔接蛋白（APPL）1水平，分析血清APPL1水平与体质量指数（BMI）、体
脂分布（WHR）、收缩压（SBP）、舒张压（DBP）、空腹血糖（FPG）、空腹血清胰岛素（FINS）、糖化血红蛋白（HbA1c）、胰岛素抵抗指
数（HOMA-IR）等的关系，初步探讨新诊断2型糖尿病患者血APPL1水平的变化。方法应用酶联免疫吸附法（ELISA）对临床上
新诊断的2 型糖尿病患者63 例（病例组）和健康对照者22 例（对照组），进行空腹血清APPL1 水平测定。稳态模型
（HOMA-MODEL）计算胰岛素抵抗指数（HOMA-IR）。结果（1）病例组血清APPL1水平（85.71±27.39）pg/ml显著高于对照组
（64.52±16.28）pg/ml，差异有统计学意义（P<0.01）；（2）2型糖尿病（T2DM）组BMI、WHR、SBP、FINS、LgHOMA-IR均高于对照
组（P<0.01），低密度脂蛋白胆固醇(LDL-C)高于对照组（P<0.05）；而T2DM组高密度脂蛋白胆固醇（HDL-C）显著低于对照组
（P<0.01）；（3）APPL1 与空腹血糖（FPG）、空腹血胰岛素（FINS）、胰岛素抵抗指数（LgHOMA-IR）呈显著正相关关系（r=0.215、
0.297、0.334，P=0.014、0.006、0.002）；（4）以APPL1为应变量进行多元线性回归分析，结果胰岛素抵抗指数（β=0.329，P=0.002）进
入方程。结论新诊断2型糖尿病患者血清APPL1水平增高，APPL1可能与2型糖尿病的发生发展密切相关。

Serum APPL1 level is elevated in newly diagnosed cases of type 2 diabetes mellitus

Objective To investigate serum APPL1 level in patients with newly diagnosed type 2 diabetes mellitus(T2DM) and analyze its correlation with body mass index(BMI),waist-to-hip ratio(WHR),blood pressure,fasting plasma glucose,fasting blood insulin,HbA1c,and insulin resistance(HOMA-IR).Method Serum APPL1 levels were determined using ELISA in 22 normal control subjects and 63 patients with newly diagnosed T2DM,and HOMA-IR of the subjects was calculated using the HOMA model.Results The diabetic patients had significantly elevated levels of serum APPL1 compared with the control subjects(85.71±27.39 vs 64.52±16.28 pg/ml,P<0.01),with also significantly increased BMI,WHR,SBP,FINS,LgHOMA-IR(P<0.01) and LDL-C(P<0.05) but lowered HDL-C(P<0.01).Fasting serum APPL1 levels were positively correlated with FPG,FINS,and HOMA-IR(r=0.215,0.297,0.334,P=0.014,0.006,0.002,respectively).In multiple linear regression analysis with APPL1 as the dependent variable,HOMA-IR(β=0.329,P=0.002) was included in the equation.Conclusion Patients with newly diagnosed T2DM have elevated serum APPL1 levels,suggesting the involvement of APPL1 in the development of T2DM.