| 表没食子儿茶素没食子酸酯的甲基化分子修饰 |
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| 引用本文： | 吕海鹏,孙业良,林 智,谭俊峰,郭 丽. 表没食子儿茶素没食子酸酯的甲基化分子修饰[J]. 食品科学, 2010, 31(15): 139-142. DOI: 10.7506/spkx1002-6630-201015031 |
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| 作者姓名： | 吕海鹏 孙业良 林 智 谭俊峰 郭 丽 |
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| 作者单位： | 中国农业科学院茶叶研究所，茶叶加工工程研究中心 |
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| 基金项目： | 浙江省自然科学基金项目(Y307498)；现代农业产业技术体系建设专项(nycytx-26)；国家自然科学基金项目(30972404) |
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| 摘 要： | 研究表没食子儿茶素没食子酸酯(EGCG)的甲基化分子修饰。以碘甲烷作为甲基供体，采用化学合成方法研究EGCG 的甲基化分子修饰，并通过HPLC-MS 和NMR 等对反应产物进行结构鉴定。结果表明：采用化学合成方法能有效完成EGCG 的甲基化分子修饰，并分离鉴定出5 个EGCG 甲基化衍生物，分别为4″-Me-EGCG、4′,4″-di-Me-EGCG、5,3′,4′,5′,3″,4″,5″-hepta-Me-EGCG、5,7,3′,4′,3″,4″,5″-hepta-Me-EGCG、5,7,3′,4′,5′,3″,4″,5″-octa-Me-EGCG。
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| 关 键 词： | 表没食子儿茶素没食子酸酯 甲基化 分子修饰 碘甲烷 化学合成 |
| 收稿时间： | 2010-03-31 |
Methylation of (-)-Epigallocatechin-3-gallate (EGCG) |
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| LU Hai-peng,SUN Ye-liang,LIN Zhi,TAN Jun-feng,GUO Li. Methylation of (-)-Epigallocatechin-3-gallate (EGCG)[J]. Food Science, 2010, 31(15): 139-142. DOI: 10.7506/spkx1002-6630-201015031 |
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| Authors: | LU Hai-peng SUN Ye-liang LIN Zhi TAN Jun-feng GUO Li |
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| Affiliation: | (Engineering Research Center for Tea Processing, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou310008, China) |
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| Abstract: | For EGCG methylation via chemosynthesis, methyl iodide was used as methyl donor and two reaction systems (No.1 and No.2) were designed, in which the molar ratios between EGCG and methyl iodide were set at 1:80 and 1:1, respectively. Reaction products TRI-1 and TRI-2 obtained from reaction system No.1 and No.2, respectively were characterized by HPLCMS and NMR. The results showed that the molecular modification of EGCG could be successfully accomplished by the use of chemosynthesis, and five EGCG derivatives, namely 4″-Me-EGCG, 4′,4″-di-Me-EGCG, 5,3′,4′,5′,3″,4″,5″-hepta- Me- EGCG, 5,7,3′,4′,3″,4″,5″-hepta- Me-EGCG and 5,7,3′,4′,5′,3″,4″,5″-octa- Me-EGCG were identified in the two reaction products, of which, 3 were contained in reaction product TRI-1 and 2 in reaction product TRI-2. |
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| Keywords: | EGCG methylation molecular modification methyl iodide chemosynthesis |
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