酸浆宿萼中β-隐黄质体外淬灭单线态氧能力评价

目的：从酸浆(Physalis alkengi L. var. franchetii (Mast.) Makino)宿萼中制备β- 隐黄质(β-cryptoxanthin)，应用微弱发光测量方法对β- 隐黄质体外淬灭单线态氧的能力进行评价。方法：用正己烷萃取酸浆宿萼中的β- 隐黄质酯后经皂化获得单体，使用C18-HPLC-PDA 对制备物进行定性和定量。用次氯酸钠- 双氧水反应产生单线态氧，使用微弱发光测量仪检测制备组分淬灭单线态氧的能力。结果：经C18-HPLC-PDA 鉴定，制备物中的类胡萝卜素主要为β- 隐黄质。微弱发光测量仪的数据显示：β- 隐黄质的存在能减少次氯酸钠- 双氧水单线态氧体系中产生的最大光子计数，且存在量效关系。0.049～0.49g/L 的β- 隐黄质可减少50% 的最大光子数。

in vitro Singlet Oxygen Quenching Ability Assessment of β-Cryptoxanthin from the Calyx of Physalis alkengi L. var. franchetii (Mast.) Makino

Objective: To assess the ability of β-cryptoxanthin from the calyx of Physalisalkengi L. var. franchetii (Mast.) Makino to quench in vitro singlet oxygen. Methods: β-cryptoxanthin eater was extracted from the calyx of Physalisalkengi L. var. franchetii (Mast.) Makino with n-hexane and then saponified to produce β-cryptoxanthin fraction. The fraction was identified and quantified on C18-HPLC-PDA. Based on the reaction between NaClO and H2O2, singlet oxygen was produced. Singlet oxygen quenching ability of the β-cryptoxanthin fraction was determined on ultra-weak luminescience analyzer. Results: By C18-HPLCPDA, the prepared fraction was identified as β-cryptoxanthin. The data from ultra-weak luminescience analyzer suggested that the β-cryptoxanthin fraction was able to reduce the maximum number of photon generated from the reaction solution in positive correlation with its amount. β-Cryptoxanthin with an amount ranging between 0.049 g/L and 0.49 g/L resulted in a 50% reduction of maximum photon number.