| 三妙丸中盐酸小檗碱的含量测定 |
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| 引用本文： | 蔡俊安. 三妙丸中盐酸小檗碱的含量测定[J]. 中国药业, 2011, 20(6): 38-39 |
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| 作者姓名： | 蔡俊安 |
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| 作者单位： | 河南百年康鑫药业有限公司,河南,周口,477150 |
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| 摘 要： | 目的采用高效液相色谱(HPLC)法测定三妙丸的盐酸小檗碱含量。方法采用Diamonsil ODS1 C18色谱柱(250 mm×4.6 mm,5μm),以50∶50的乙腈-0.1%磷酸溶液(每100mL加十二烷基磺酸钠0.1 g)为流动相,流速为1.0 mL/min,检测波长265 nm。结果盐酸小檗碱进样量在0.127～2.54μg范围内与峰面积线性关系良好,回归方程Y=418 567.505-92 087.201X,r=0.999 4。平均加样回收率为99.53%,RSD为0.48%(n=6)。结论该法简便、准确、专属、重复性好,为三妙丸的盐酸小檗碱含量分析提供了科学有效的方法。
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| 关 键 词： | 三妙丸 盐酸小檗碱 高效液相色谱法 含量 |
Determination of Berberine Hydrochloride in Sanmiao Pills by HPLC |
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| Cai Jun'an. Determination of Berberine Hydrochloride in Sanmiao Pills by HPLC[J]. China Pharmaceuticals, 2011, 20(6): 38-39 |
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| Authors: | Cai Jun'an |
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| Affiliation: | Cai Jun'an(Henan Bainian Kangxin Pharmaceutical CO.,Ltd.,Dancheng,Henan,China 477150) |
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| Abstract: | Objective To establish a HPLC method for determining the content of berberine hydrochloride in Sanmiao Pills.Methods The Diamonsil ODS1 C18 column was adopted with acetonitrile-0.1% phosphoric acid solution（50 ∶50）（adding 0.1 g sodium dodecyl sulfate per 100 mL solution）as the mobile phase.The detection wavelength was 265 nm and the flow rate was 1.0 mL/min.Results The calibration curve was linear in the range of 0.127-2.54 μg for berberine hydrochloride and the regression equation was Y=418 567.505-92 087.201X,r=0.999 4.The average recovery rate was 99.53%,RSD=0.48%（n=6）.Conclusion This method is simple,accurate and specific with good reproducibility,which can provide the scientific and effective content analysis method of berberine hydrochloride in Sanmiao Pills. |
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| Keywords: | Sanmiao Pills berberine hydrochloride HPLC content |
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