参芪降糖颗粒中非法添加格列本脲的检测

目的:建立降糖中药制剂参芪降糖颗粒中非法添加格列本脲的检测方法。方法:采用薄层色谱法进行鉴别;采用高效液相色谱法测定格列本脲的含量:色谱柱为Kromasil C18(250mm×4.6mm,5μm),流动相为磷酸二氢铵溶液(用磷酸调pH3.15)-甲醇(25:75),检测波长为240nm。结果:格列本脲的检测浓度在0.0301～0.4816mg.mL-1范围内与峰面积积分值呈良好的线性关系(r=0.9992);平均回收率为98.62%,RSD=0.54%(n=9)。结论:本方法灵敏可靠、简单可行,可用于降糖中药制剂中非法添加格列本脲的检测。

Detection of Glibenclamide Mixed Illegally in Shenqi Hypoglycemic Granules

OBJECTIVE： To establish a method for detection of glibenclamide mixed illegally in Shenqi hypoglycemic granules, a hypoglycemic traditional Chinese medicine preparation.METHODS： The constituents were identified by TLC, and the content of glibenclamide was determined by HPLC on Kromasil C18 column（250 mm × 4.6 mm, 5μm） with a ammonium dihydrogen phosphate（adjusted pH = 3.15 with phospharic acid） -methanol（25 ： 75） as the mobile phase .The detection wavelength was set at 240 nm. RESULTS： The linear range of glibenclamide was 0.030 1-0.481 6 mg · mL^-1 （r = 0.999 2） with an average recovery at 98.62% （RSD = 0.54%, n = 9） .CONCLUSION： The method is sensitive and reliable, simple and feasible, and suitable for the detection of glibenclamide mixed illegally in hypoglycemic traditional Chinese medicine preparations.